实验动物与比较医学2018，Vol.38Issue(2)：91-97,7.DOI:10.3969/j.issn.1674-5817.2018.02.003
啮齿类螺杆菌SYBR Green Ⅱ荧光定量PCR检测方法的建立
Establishment of SYBR Green Ⅱ Based Real-time PCR Method for Detection of Helicobacter Rodentium
摘要
Abstract
Objective To establish a rapid detection method for helicobacter rodentium.Method A pair of specific primers was designed according to the gene sequence of Helicobacter rodentium 16S rRNA.The method of SYBR Green Ⅱ real-time PCR was established.Results The standard curve cycle threshold had a good linear relationship with the template concentration in the range of 102 copies/μL to 107 copies/μL concentration.This method has good sensitivity,only specific amplification of Helicobacter rodentium,could detect 30 copies/μL,the coefficient of variation (CV) was less than 2％ within and between groups with good reproducibility.Conclusion The method of SYBR Green Ⅱ realtime PCR was established for the identification of Helicobacter rodentium.关键词
啮齿类螺杆菌/SYBR Green Ⅱ/荧光定量PCRKey words
Helicobacter rodentium/SYBR Green Ⅱ/Real-time PCR分类
生物科学引用本文复制引用
孙靖谕,谢灵志,冯洁,于宁,钱淼,曹舒扬,张泉..啮齿类螺杆菌SYBR Green Ⅱ荧光定量PCR检测方法的建立[J].实验动物与比较医学,2018,38(2):91-97,7.基金项目
上海市科技创新行动计划(17140900600) （17140900600）
