实验动物与比较医学2018，Vol.38Issue(2)：98-104,7.DOI:10.3969/j.issn.1674-5817.2018.02.004
牛副流感病毒3型RT-PCR方法的建立及初步应用
Establishment and Preliminary Application of RT-PCR Method for Detection of Bovine Parainfluenza Virus Type3 in Bovine Origin Samples
摘要
Abstract
Objective To establish a RT-PCR method for detection of bovine parainfluenza virus type3 (BPIV3) in bovine origin samples.Methods The primers were designed and synthesized according to the published BPIV3 specific sequences of neuraminidase (HN) gene.RT-PCR method was established,and the specificity,sensitivity,repeatability and stability of this method were tested.Then the method was used to detect 137 bovine origin samples.Results The developed RT-PCR method was no cross reaction with bovine viral diarrhea virus (BVDV),bovine herpesvirus type1(BHV-1),Sendai virus (SV).The lowest detection virus titer is 106/mL.BPIV3 cDNA maintained at-30 ℃ for 12 months,still can enlarge the fragment of about 610 bp visible purpose belt.The nucleic acid positive rate of 137 bovine origin samples were 14.6％ about BPIV3 detected by RT-PCR.Conclusion The developed RT-PCR method is good in specificity,sensitivity,stability,and can be used for detecting the BPIV3 in bovine origin samples.关键词
牛副流感病毒3型(BPIV3)/PCR/牛源性样本Key words
Bovine parainfluenza virus type3 (BPIV3)/PCR/Bovine origin sample分类
医药卫生引用本文复制引用
王吉,贺争鸣,付瑞,李晓波,王淑菁,王莎莎,李威,秦骁,巩薇,岳秉飞..牛副流感病毒3型RT-PCR方法的建立及初步应用[J].实验动物与比较医学,2018,38(2):98-104,7.基金项目
中国食品药品检定研究院学科带头人培养基金项目(2015X5) （2015X5）
