实验动物与比较医学2018，Vol.38Issue(2)：111-116,6.DOI:10.3969/j.issn.1674-5817.2018.02.006
硒化卡拉胶对甲型肝炎减毒活疫苗诱导的小鼠体液免疫应答的影响
Effect of Kappa-selenocarrageenan on Humoral Immune Response Induced by Hepatitis a Virus Attenuated Live Vaccine in Mice
摘要
Abstract
Objective To investigate the effect of kappa-selenocarrageenan (KSC) on humoral immune response induced by hepatitis A virus attenuated live vaccine (HepA-1) in mice.Methods Seven experiment groups were set up,with 7 ICR mouse per group randomly,negative control group (saline),pure antigen group (18EU HepA-1),Aluminium adjuvant group [Al(OH)3 0.3 mg+18 EU HepA-1],KSC experimental groups (KSC 1 mg+18 EU HepA-1,KSC 0.5 mg+18 EU HepA-1,and KSC 0.25 mg +18 EU HepA-1,KSC 0.1 mg+18 EU HepA-1),in which mice were injected subcutaneously with 300 μL,immunizing one time.The anti-HAV specific IgG antibody levels were tested by indirect ELISA method in the serum of mice after the first 4th,8th,12th,16th week of injection.The healthy status of mice were observed during the study.The kidney,spleen,liver,lung and heart of mice immunized with KSC+HepA-1 at the optimal dosage and those in negative control group were taken out at 16th week after immunization,and prepared into sections for pathological observation.Results HAV-specific IgG antibody of all mice were detected at four setting time point except negative control group.The antibody level of all groups increased first and then decreased.The peak of Aluminium adjuvant group at the 12th week and others at the 8th week.The level of antibody in KSC 0.5 mg+18EU HepA-1 group at 8th,12th week was higher than that in simple antigen group (t=3.103,t=2.573,P＜0.05).The level of antibody in KSC 0.25 mg +18EU HepA-1 group at week 12 was higher than that in pure antigen group(t=-2.602,P＜0.05).The optimal dosage of mice in this test was 0.5 mg.No abnormality was observed in the physiological indexes of mice in various groups,while there was no pathological changes in the kidney,spleen,liver,lung and heart of mice immunized with HepA-1 +0.5 mg KSC.Conclusion KSC can be used as an adjuvant to enhance humoral immune response induced by HepA-1 in mice.关键词
硒化卡拉胶(KSC)/佐剂/甲型肝炎减毒活疫苗(HepA-1)/体液免疫Key words
Kappa-selenocarrageenan(KSC)/Adjuvant/Hepatitis A virus attenuated live vaccine (HepA-1)/Humoral immune分类
生物科学引用本文复制引用
贾森泉,金晓,李晓红,王海璇,胡凝珠,胡云章..硒化卡拉胶对甲型肝炎减毒活疫苗诱导的小鼠体液免疫应答的影响[J].实验动物与比较医学,2018,38(2):111-116,6.基金项目
国家重点研发计划“高等级病原微生物实验室模拟培训体系研究”(2016YFC1202305) （2016YFC1202305）
中国医学科学院医学与健康科技创新工程(2017-12M-3-022) （2017-12M-3-022）
国家自然科学基金(31500724 （）
31500748) （）
云南省重大科技专项(2017ZF007) （2017ZF007）
