同济大学学报（医学版）2018，Vol.39Issue(2)：12-16,5.DOI:10.16118/j.1008-0392.2018.02.003
IL-21及其受体在银屑病患者外周血和皮损组织中的表达及意义
Expression of IL-21 and IL-21R in peripheral blood mononuclear cells and skin tissue of patients with psoriasis
摘要
Abstract
Objective To investigate the expression of IL-21 and IL-21R in peripheral blood and skin tissues of patients with psoriasis vulgaris. Methods The expression of IL-21 and IL-21R in peripheral blood mononuclear cells(PBMCs)of 58 patients with psoriasis and 20 healthy controls were detected by flow cytometry. The expression of IL-21 and IL-21R mRNA and protein in PBMCs and skin tissues of 25 psoriasis patients and 15 non-psoriasis patients(control)was detected by Real-time PCR and Western blotting,respectively. The levels of IL-21 in the PBMCs culture supernatant were detected by ELISA. Results The flow cytometry showed that percentages of positive IL-21 and IL-21R CD4+T cells was significantly increased in patients with psoriasis. Real-time PCR and Western blotting showed that the expressions of IL-21 and IL-21R mRNA and protein in psoriasis patients were significantly higher in PBMCs and skin tissues than those in controls.ELISA detection showed that the IL-21 secretion level in PBMCs culture supernatant of psoriasis patients was increased.Conclusion Expressions of IL-21 and IL-21R in PBMCs and skin tissues in psoriasis patients are higher than in healthy controls.IL-21 may be involved in the pathogenesis of psoriasis,and maybe a potential therapeutic target.关键词
银屑病/白细胞介素21/白细胞介素21受体Key words
psoriasis/interleukin-21/interleukin-21 receptor分类
医药卫生引用本文复制引用
范华宇,龚瑜,于倩,史玉玲..IL-21及其受体在银屑病患者外周血和皮损组织中的表达及意义[J].同济大学学报（医学版）,2018,39(2):12-16,5.基金项目
国家自然科学基金(81673050,81301356) （81673050,81301356）
上海市自然科学基金(16ZR1426800) （16ZR1426800）
