同济大学学报（医学版）2018，Vol.39Issue(2)：32-37,6.DOI:10.16118/j.1008-0392.2018.02.007
靶向整合素αvβ3受体磁共振/荧光双模态成像纳米探针的体外成像性能及光动力治疗效应评价
Magnetic resonance imaging and photodynamic therapeutic effects in vitro of nanoprobe RGD-Gd@BSA-Ce6 targeting αvβ3 receptor
摘要
Abstract
Objective To evaluate the imaging and photodynamic therapy treatment(PDT)efficacy of nanoprobe RGD-Gd@BSA-Ce6 on non-small cell lung cancer(NSCLC)A549 cells. Methods The protein nanoprobe RGD-Gd@BSA-Ce6 and Gd@BSA-Ce6 were synthesized. The T1relaxation time of RGD-Gd@BSA-Ce6 and Gd-DTPA was collected by in vitro magnetic field generator and both T1-weighted images were attained by using 3.0T magnetic resonance scanner.The affinity of the chlorine6(Ce6),Gd@BSA-Ce6 and RGD-Gd@BSA-Ce6 with A549 cells was observed by laser confocal fluorescence imaging, the PDT effect was determined by CCK-8 assay(cell counting Kit-8). The biosecurity of three drugs to human embryonic kidney 293T cells was assessed by MTT assay. Results RGD-Gd@BSA-Ce6 showing relaxation coefficient 18.615 had higher T1relaxation rate and T1-weighted enhanced imaging signal compared with Gd-DTPA.More distinct property of affinity with A549 cells was observed in group RGD-Gd@BSA-Ce6 and group Gd@BSA-Ce6 by laser confocal fluorescence imaging,both were better than free Ce6,and RGD-Gd@BSA-Ce6 showed higher affinity with A549 cells than Gd@BSA-Ce6. PDT tests showed that relative cell viability in RGD-Gd@ BSA-Ce6 and Gd@ BSA-Ce6 groups were significantly lower than that in Ce6 group(P<0.05);while the cell viability in RGD-Gd@BSA-Ce6 group was lower than that in Gd@BSA-Ce6 group(P<0.05).The biosecurity assay in vitro showed that there were no significant changes in 293T cells under different drug concentrations,and relative cell viability was all higher than 60%.Conclusion RGD-Gd@BSA-Ce6 has a better T1relaxation performance than commercial Gd-DTPA.In vitro experiments proves that RGD-Gd@BSA-Ce6 protein nanoprobe has a high affinity with A549 cell and significant PDT effect on A549 cell with a good biosecurity.关键词
肺肿瘤/整合素αvβ3受体/精氨酸-甘氨酸-天冬氨酸/磁共振/荧光成像/光动力治疗Key words
lung cancer/integrin receptor/arginine-glycine-aspartate/MRI/Fluorescence imaging/photodynamic therapy treatment分类
医药卫生引用本文复制引用
张旭南,王培军..靶向整合素αvβ3受体磁共振/荧光双模态成像纳米探针的体外成像性能及光动力治疗效应评价[J].同济大学学报（医学版）,2018,39(2):32-37,6.基金项目
国家自然科学基金(81571655) （81571655）
