同济大学学报（医学版）2018，Vol.39Issue(2)：38-44,7.DOI:10.16118/j.1008-0392.2018.02.008
妊娠期糖尿病胎盘基因表达变化及功能分析
Placental gene-expression profile in gestational diabetes mellitus
摘要
Abstract
Objective To explore the differentially expressed genes(DEGs)and their function in placenta of patients with gestational diabetes mellitus(GDM). Methods Placenta samples were obtained from 3 normal puerperants and 3 puerperants with GDM who underwent cesarean section for delivery. Microarray chips were used to screen the DEGs in GDM placenta followed by Real-time quantitative PCR(RT-qPCR)validation. Gene GO and KEGG database were employed to analyze the function of DEGs. Results Total 1 773 genes(4.3%)were found to be differentially expressed in GDM placenta(P<0.05). Gene Go analysis revealed DEGs function in the aspects of biological process, molecular function and cellular component. The biological process mainly was related to regulation of DNA transcription,protein phosphorylation and gene expression. The molecular function was related to protein binding, metal ion binding and zinc ion binding. The cellular components of DEGs were mainly in nucleus,cytoplasm and intracellular fluid. Gene KEGG analysis showed that the top three signaling pathways were Wnt signaling pathway, Spliceosome, and TGF-β signaling pathway. Conclusion There are some gene expressed differentially in placenta of patients with GDM, which are involved in various biological process, cellular components and molecular function. The study result would be of value in the understanding and manage of gestational diabetes mellitus.关键词
妊娠期糖尿病/基因芯片/胎盘/基因表达Key words
gestational diabetes mellitus/microarray/placenta/gene expression分类
医药卫生引用本文复制引用
倪晓田,黄小杰,王凯,周健..妊娠期糖尿病胎盘基因表达变化及功能分析[J].同济大学学报（医学版）,2018,39(2):38-44,7.基金项目
上海市自然科学基金(14ZR1433000) （14ZR1433000）
上海市浦东新区联合攻关项目(PW2014D-8) （PW2014D-8）
