同济大学学报（医学版）2018，Vol.39Issue(2)：45-50,6.DOI:10.16118/j.1008-0392.2018.02.009
POFUT1对人胃腺癌裸鼠移植瘤微血管生成的抑制作用
POFUT1 inhibits angiogenesis of human gastric adenocarcinoma xenografts in nude mice
摘要
Abstract
Objective To investigate the effect of POFUT1 on angiogenesis of human gastric adenocarcinoma xenografts in nude mice. Methods Human gastric adenocarcinoma BGC823 cells were transfected with shRNA-POFUT1, shRNA control, PLV-POFUT1 and PLV contro1, respectively. The transfected BGC823 cells were inoculated in nude mice. The volume of transplanted tumors was measured and the tumor growth curve was generated. The tumor-bearing nude mice were sacrificed and the tumors were harvested 30 days after transplantation. The expression of VEGF and CD31 was determined by immunohistochemistry and the expression of POFUT1 and VEGF protein was detected by Western blotting. Results Twenty tumor-bearing nude mice were in good condition during the experiments. Compared with PLV control group,the volume of tumor in PLV-POFUT1 group was increased significantly(P < 0.05); whereas the tumor volume in shRNA-POFUT1 group was significantly lower than that in shRNA control group(P<0.05). Immunohistochemistry and Western blotting showed that the expression levels of VEGF and CD31 in PLV-POFUT1 group were significantly higher than those in control group(P<0.05), while the expression levels of POFUT1, VEGF and CD31 in the ShPOFUT1 group were significantly lower than those in control group(P<0.05). Conclusion Over-expression of POFUT1 significantly promotes the proliferation and angiogenesis of transplanted human gastric edunocarcinoma in nude mice, while low expression of POFUT1 can inhibit the proliferation and tumor angiogenesis, which indicates that POFUT1 may play an important role in the angiogenesis of human gastric adenocarcinoma xenografts, which may be closely related to VEGF.关键词
胃肿瘤/蛋白-O-岩藻糖基转移酶1/血管内皮细胞生长因子/微血管密度/裸鼠Key words
gastric cancer/protein-O-fucosyltransferase1/vascular endothelial cell growth factor/microvessel density/nude mice分类
医药卫生引用本文复制引用
樊启林,王志荣..POFUT1对人胃腺癌裸鼠移植瘤微血管生成的抑制作用[J].同济大学学报（医学版）,2018,39(2):45-50,6.基金项目
上海市科学技术委员会重大科技项目(1312JC1408402) （1312JC1408402）
