同济大学学报（医学版）2018，Vol.39Issue(2)：51-55,5.DOI:10.16118/j.1008-0392.2018.02.010
乌司他丁对脓毒症大鼠胰腺外分泌功能的保护作用及机制研究
Protective effect of ulinastatin on pancreatic exocrine function in rats with sepsis and related mechanisms
摘要
Abstract
Objective To investigate the effect of ulinastatin on pancreatic exocrine function in rats with sepsis and related mechanisms. Methods Thirty male SPF rats were randomly divided into control group,LPS group and UTI group with 10 animals in each group. The sepsis model was induced by intraperitoneal injection of LPS(15 mg/kg). Intraperitoneal injection of ulinastatin(50 000 U/kg)was given to rats in UTI group when sepsis model was established, while same volume of normal saline was given to rats in LPS group and control group. Blood samples were collected 12 h after sepsis model was made,and the level of amylase and lipase were determined by automatic biochemical analyzer,TNF-α was determined by enzyme-linked immunosorbent assay(ELISA).The morphological changes of pancreas tissue were observed by H-E staining. The apoptosis of acinar cells was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling(TUNEL)assay. The expression of apoptosis factors(CytC, Caspase3,Bcl-2 and Bax)were determined by Western blotting.Results Serum amylase,lipase and TNF-α levels in LPS group and UTI group were significantly higher than those in control group(P<0.01),while the levels in UTI group was significantly lower than those in LPS group(P<0.01). The H-E staining of pancreatic tissue showed that the modified Schmidt scores of control group,LPS group and UTI group were 0,5.13 and 3.70 respectively(P<0.01). The apoptosis index in LPS group was significantly higher than that in control group(P<0.01),the index in UTI group was significantly lower than that in LPS group(P<0.01).The expressions of CytC, Caspase3 and BAX in LPS group were significantly higher than that in control group,the expressions in UTI group were significantly lower than that in LPS group;while the expression of Bcl-2 in LPS group was significantly lower than that in control group,the expression in UTI group was significantly higher than that in LPS group. Conclusion Pancreatic exocrine function is damaged in septic rats induced by LPS. Ulinastatin can protect the pancreatic exocrine function in rats with sepsis,which is associated with the inhibition of apoptosis.关键词
乌司他丁/脓毒症/胰腺外分泌/大鼠Key words
ulinastatin/sepsis/exocrine pancreatic/rats分类
医药卫生引用本文复制引用
陆志峰,赵丽,王胜..乌司他丁对脓毒症大鼠胰腺外分泌功能的保护作用及机制研究[J].同济大学学报（医学版）,2018,39(2):51-55,5.基金项目
天普研究基金(UF201408) （UF201408）
