同济大学学报（医学版）2018，Vol.39Issue(2)：56-60,5.DOI:10.16118/j.1008-0392.2018.02.011
淫羊藿苷对白介素-1β诱导软骨细胞退变的保护作用
Icariinin protects chondrocytes from IL-1β-induced degeneration
摘要
Abstract
Objective To investigate the effect of icariin(ICA)on IL-1β-induced chondrocytes degeneration. Methods Primary chondrocytes isolated from articular cartilage of newborn rats were cultured in vitro and treated with icariin at 1×10-7, 1×10-6and 1×10-5mol/L. The cell proliferation was measured by MTT at 12, 24, 36, 48, 60 and 72 h. The cultured rat chondrocytes were than dividing into control group(N group), IL-1β-stimulated group(M group treated with IL-1β 10 ng/ml), ICA-L(1×10-7mol/L)and ICA-H(1×10-6mol/L)groups(IL-1β-stimulated cells treated with ICA). The concentrations of collagen-Ⅱ and glycosaminoglycan(GAG)in culture media after 72 h of intervention were determined by ELISA and the expressions of Col-Ⅱ,MMP13,Acan,Sox9 mRNA were detected by RT-PCR. Results 1×10-5mol/L ICA inhibited chondrocytes proliferation when intervened for 24 h,while promoted cell proliferation after 72 h;there was no significant difference between groups with and without ICA at 48 h or 60 h.ICA concentration at 1×10-7mol/L and 1×10-6mol/L both significantly promoted cell proliferation from 36 h to 72 h, but ICA at 1×10-7mol/L had no significant effect at 60 h.ICA at both concentration of 1×10-7mol/L and 1×10-6mol/L increased the GAG level in culture supernatant,but there was no significant change in Col-Ⅱlevel in ICA-H group. Compared with M group, the expression of Acan/Sox9 mRNA in ICA-L group and Col-ⅡmRNA in ICA-H group significantly went up,while MMP13 decreased in ICA-H group. Conclusion Icariin can affect the matrix microenvironment of chondrocytes, promote the phenotype gene expression and inhibit MMP13 mRNA expression,and further protects chondrocytes from IL-1β-induced degeneration.关键词
淫羊藿苷/软骨细胞/软骨退变/骨关节炎/大鼠Key words
icariin/chondrocytes/cartilage degeneration/osteoarthritis/rat分类
医药卫生引用本文复制引用
刘益杰,陈世宣,赵仙丽,宁长青,冯伟..淫羊藿苷对白介素-1β诱导软骨细胞退变的保护作用[J].同济大学学报（医学版）,2018,39(2):56-60,5.基金项目
上海市自然科学基金(15ZR1441000) （15ZR1441000）
