同济大学学报（医学版）2018，Vol.39Issue(2)：61-65,72,6.DOI:10.16118/j.1008-0392.2018.02.012
ZIC5在前列腺癌中的表达及意义
Effect of ZIC5 on biological behaviors of prostate cancer and its mechanisms
张瑜 1奚雪滔 1赵雪1
作者信息
- 1. 上海交通大学医学院附属同仁医院泌尿外科,上海 200336
- 折叠
摘要
Abstract
Objective To investigate the expression of ZIC5 in prostate cancer and its effect on the biological behavior of prostate cancer. Methods Expression of ZIC5 in prostate cancer samples and prostate cancer PC3 and LNCaP cells were detected by quantitative real-time PCR(qRT-PCR). Cell proliferation and migration were determined by CCK-8 and Transwell methods after PC3 and LNCaP cells transfected with ZIC5 siRNA. Bioinformatics was used to predict the upregulated microRNA,and the target gene was verified by dual-luciferase reporter assay. Results Expression of ZIC5 was upregulated in prostate cancer samples compared with adjacent normal tissues(t=4.2,P<0.001),also elevated in prostate cancer PC3 and LNCaP cells(P<0.05). CCK-8 and Transwell assays showed that cell proliferation,migration and invasion in PC3 and LNCaP cells were inhibited 24 h after transfected with ZIC5 siRNA. Bioinformatics and dual-luciferase assay showed that ZIC5 was the target gene of miR-449 family. The expression level of ZIC5 was negatively correlated with the expression of miR-449 family in human prostate cancer samples(ZIC5 vs miR-449a: r=-0.64,P<0.05;ZIC5 vs miR-449b,r=-0.52,P<0.05).Over-expression of ZIC5 activated epithelial-mesenchymal transition(EMT)of prostate cancer cells. Knock-down of ZIC5 and restoration of miR-449 family reversed the ZIC5-activated EMT in prostate cancer. Conclusion Aberrant expression of ZIC5 promotes the growth and invasion of prostate cancer and it is regulated by miR-449 family.关键词
ZIC5/前列腺肿瘤/增殖/侵袭Key words
zic family member 5/prostate cancer/proliferation/invasion分类
医药卫生引用本文复制引用
张瑜,奚雪滔,赵雪..ZIC5在前列腺癌中的表达及意义[J].同济大学学报（医学版）,2018,39(2):61-65,72,6.
