同济大学学报（医学版）2018，Vol.39Issue(2)：73-78,6.DOI:10.16118/j.1008-0392.2018.02.014
miRNA-146b-3p在早期非小细胞肺癌中的诊断价值
Diagnostic value of miRNA-146b-3p in early non-small cell lung cancer
摘要
Abstract
Objective To assess the diagnostic value of miR-146b-3p in peripheral blood for early non-small cell lung cancer(NSCLC). Methods Peripheral blood samples were collected from 118 patients with early NSCLC, 42 patients with benign pulmonary nodules and 48 healthy subjects. Circulating miR-15b-5p, miR-17-5p, miR-19a-3p, miR-20a-5p, miR-92a-3p and miR-146b-3p were detected by real-time fluorescent quantitative PCR(RT-qPCR)and compared among different groups. Results The expression of miR-146b-3p was significantly higher in NSCLC patients than that in patients with benign lung nodules and healthy controls. The area under the ROC curve(AUC)of miR-146b-3p in differential diagnosis between NSCLC and benign lung nodules was 0.77;with the cut-off value of 0.01,the sensitivity and specificity was 75% and 71%,respectively. The AUC of miR-146b-3p in differentiating early NSCLC patients from healthy subjects was 0.93; with the cut-off value of 0.72,the sensitivity and the specificity was 89% and 84%, respectively. Logistic regression model indicated that age and miR-146b-3p were independent risk factors for early NSCLC compared to benign lung nodules,and the AUC was 0.81,with the cut-off value of 0.85,the sensitivity and the specificity was 65% and 89%. MiR-19a-3p and miR-146b-3p were independent risk factors for early NSCLC compared to healthy controls,and the AUC was 0.98, with the cut-off value of 0.90, the sensitivity and specificity was 94% and 95%, respectively. Conclusion MiR-146b-3p can differentiate early NSCLC from benign lung nodules and healthy control, indicating that it might be used as a new biomarker for early diagnosis of non-small cell lung cancer.关键词
非小细胞肺肿瘤/miRNAs/miR-146b-3pKey words
non small cell lung cancer/miRNAs/miR-146b-3p分类
医药卫生引用本文复制引用
沙杰,范理宏..miRNA-146b-3p在早期非小细胞肺癌中的诊断价值[J].同济大学学报（医学版）,2018,39(2):73-78,6.基金项目
上海市科委纳米专项基金(12NM0500800) （12NM0500800）
同济大学"中央高校基本科研业务费-重点学科交叉类项目"(1501219108) （1501219108）
