水生生物学报2018，Vol.42Issue(3)：451-462,12.DOI:10.7541/2018.057
基于G-SSR和EST-SSR标记的鲫6个群体遗传结构分析
GENETIC DIVERSITY AND STRUCTURE OF CRUCIAN CARP(CARASSIUS AURATUS)BASED ON G-SSR AND EST-SSR MARKERS
摘要
Abstract
This study investigated genetic diversity and genetic structure of six wild crucian carp populations collected from Heilongjiang River, Yangtze River, Fenghua River and Huaihe River by 8 genome microsatellites (G-SSR) and 8 transcriptome microsatellites(EST-SSR).The mean Na,Ne,Ho,Heand PIC of the eight G-SSR loci were 22,12.9, 0.769, 0.893 and 0.879, respectively. Fstvalues ranged from 0.008 to 0.085, indicating that there were different degrees of genetic differentiation among the six wild crucian carp populations, among which two populations from the Hei-longjiang River system were different from all the others. There was no significant genetic differentiation among the four populations in the Yangtze River, Fenghua River and Huaihe River. The Nei's genetic distances of six wild cru-cian carp populations were between 0.203—0.701. UPGMA cluster analysis and Bayesian analysis supported that six crucian carp populations could be divided into two large branches with two populations from Heilongjiang water sys-tem as one group while the others as another group.The average Na,Ne,Ho,Heand PIC of the eight EST-SSR loci were 19, 9.5, 0.728, 0.870 and 0.855 respectively. The Fstvalues and Nei's genetic distances among the six populations were between 0.005—0.084 and 0.117—0.683, respectively. Genetic differentiation analysis, UPGMA cluster analysis and Bayesian analysis based on EST-SSR markers also separated six populations of crucian carp into two major groups: the Heilongjiang water system group as well as the Yangtze River, Fenghua River and the Huaihe River system group. Based on G-SSR and EST-SSR markers,the average polymorphism information content(PIC)of the six crucian carp populations ranged between 0.786—0.864 and 0.761—0.833, respectively, indicating that all the six wild crucian carp populations had a high genetic diversity. Although the polymorphism of the EST-SSR markers was slightly lower than that of the G-SSR markers, the two types of SSR markers revealed similar genetic structure and pattern of differentia-tion for crucian carp in three major waters of China. This study provides basic and new information for the conserva-tion of crucian carp germplasm resources and the evaluation of EST-SSR markers in genetic studies of fish populations.关键词
鲫/G-SSR/EST-SSR/遗传多样性/遗传结构Key words
Crucian carp(Carassius auratus)/G-SSR/EST-SSR/Genetic diversity/Genetic structure分类
生物科学引用本文复制引用
甘宝江,庞美霞,俞小牧,童金苟..基于G-SSR和EST-SSR标记的鲫6个群体遗传结构分析[J].水生生物学报,2018,42(3):451-462,12.基金项目
中国科学院先导专项A项目课题(XDA08010405-2) （XDA08010405-2）
淡水生态与生物技术国家重点实验室课题(2016FBZ05)资助[Supported by the Special Fund for Strategic Pilot Technology of Chinese Academy of Sciences(XDA08010405-2) （2016FBZ05）
State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)] （2016FBZ05）
