水生生物学报2018，Vol.42Issue(3)：463-472,10.DOI:10.7541/2018.058
黄颡鱼Wnt家族4个基因的克隆、组织表达及对铜的响应
CLONING AND TISSUE EXPRESSION OF FOUR WNT GENES IN YELLOW CATFISH PELTEOBAGRUS FULVIDRACO AND THEIR RESPONSE TO COPPER EXPOSURE
摘要
Abstract
Four full length Wnt cDNA sequences,designated Wnt5a,Wnt5b,Wnt7a and Wnt9b,were amplified by RT-PCR and RACE approaches from yellow catfish Pelteobagrus fulvidraco.The validated cDNAs of Wnt5a,Wnt5b, Wnt7a and Wnt9b were 1984,2905,2158 and 1622 bp in length,encoding peptides of 375,375,350 and 358 amino acid residues, respectively. The amino acid alignment and phylogenetic analysis revealed that Wnt sequences were rela-tively conserved among different vertebrates.All Wnt mRNAs were expressed in tested tissues(brain,spleen,kidney, gill, heart, muscle, mesenteric fat, liver and ovary) but with variable levels. Cu exposure for 28 days highly induced Wnt7a mRNA levels only at 30 μg Cu/L group.Cu exposure had no impact on mRNA levels of Wnt5a,Wnt5b and Wnt9b at day 28.On 56-day Cu exposure,mRNA levels of Wnt5b were not lowest at 60 μg Cu/L group and Wnt9b were the highest at 30 μg Cu/L group.Wnt5a and Wnt7a showed no significant differences among three groups.The present study indicated that functional differentiation exists in members of Wnt family, and some members may me-diate the effect of Cu exposure on ovary development of yellow catfish.关键词
黄颡鱼/Wnt/分子特征/组织表达/铜暴露Key words
Pelteobagrus fulvidraco/Wnt/Molecular characterization/Tissue distribution/Copper exposure分类
生物科学引用本文复制引用
张丽晗,罗智,有文静,李丹丹,吴坤,徐异桓..黄颡鱼Wnt家族4个基因的克隆、组织表达及对铜的响应[J].水生生物学报,2018,42(3):463-472,10.基金项目
国家自然科学基金优秀青年基金(31422056)资助[Supported by the National Natural Science Foundation of China(31422056)] （31422056）
