水生生物学报2018，Vol.42Issue(3)：473-479,7.DOI:10.7541/2018.059
大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究
CLONING AND FUNCTIONAL STUDY OF PGRP-S IN CHINESE GIANT SALAMANDER ANDRIAS DAVIDIANUS
摘要
Abstract
Peptidoglycan recognition proteins (PGRPs) are a class of pattern recognition receptors that are highly con-served from invertebrates to mammals. So far, PGRP is rarely reported in vertebrate species except for bony fish and mammals. This study constructed the eukaryotic expression plasmid of PGRP-S, a short peptidoglycan recognition pro-tein in Chinese giant salamander, and explored its functins. Sequence analysis showed that the N-terminal of PGRP-S had no signal peptide, but had two closely spaced cysteine residues and two Zn2+binding sites. Moreover, PGRP-S could be secreted to the exterior and also be retained within the cells. In vitro antibacterial experiments revealed that overexpression of PGRP-S significantly inhibited the proliferation of Edwardsiella tarda in HEK293T cells intracellu-larly and extracellularly. In addition, overexpression of PGRP-S enhanced NF-κB promoter activity and bound Lys-type and Dap-type peptidoglycan but without degradation ability.These results indicate that the Chinese giant salaman-der PGRP-S is functionally similar to mammalian PGRP-S.关键词
PGRP-S/真核表达/NF-κB/抗菌活性/肽聚糖结合活性/酰胺酶活性/大鲵Key words
PGRP-S/Eukaryotic expression/NF-κB/Antibacterial activity/Peptidoglycan binding activity/Amidase activity/Chinese giant salamander分类
生物科学引用本文复制引用
任诗思,齐志涛,昌鸣先..大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究[J].水生生物学报,2018,42(3):473-479,7.基金项目
国家自然科学基金(31172408)资助[Supported by the National Natural Science Foundation of China(31172408)] （31172408）
