水生生物学报2018，Vol.42Issue(3)：488-493,6.DOI:10.7541/2018.061
文蛤SRBI基因克隆及其在不同壳色群体中的差异表达
CLONING AND EXPRESSION ANALYSIS OF SRBI GENE IN DIFFERENT SHELL-COLOR STRAINS OF MERETRIX MERETRIX
摘要
Abstract
To explore the molecular structure and biological function of SRBI in the process of carotenoids accumula-tion in Meretrix meretrix,we cloned cDNA of Mm-SRBI by SMART RACE technique and conducted the bioinforma-tics,expression analysis and intron amplification.The full length cDNA of Mm-SRBI is 1723 bp that contains a com-plete 1515 bp ORF encoding 504 amino acids with one functional domain of Mm-SRBI protein(CD36).Mm-SRBI is highly homologous with Mimachlamys nobilis and shares 55% similarity in amino acid sequence.It shares 34%—40% similarity with vertebrates.We also amplified 12 introns of Mm-SRBI,which followed the principle of GT-AG.Mm-SRBI gene expressed in all six tested tissues with the highest level in mantle(P<0.01),suggesting that Mm-SRBI gene maybe relate to high carotenoid in mantle.Moreover,the expression of Mm-SRBI gene in red shell strain was signifi-cantly higher than that of white shell strain (P<0.05). In conclusion, this study provides helpful foundation to explore the shell colors on this species.关键词
文蛤/SRBI/基因克隆/内含子/壳色Key words
Meretrix meretrix/SRBI/Gene cloning/Intron/Shell color分类
农业科技引用本文复制引用
崔宝月,董迎辉,赵家熙,胡凌威,李晓英,姚韩韩,林志华..文蛤SRBI基因克隆及其在不同壳色群体中的差异表达[J].水生生物学报,2018,42(3):488-493,6.基金项目
国家自然科学基金(31772846) （31772846）
浙江省自然科学基金(Y16C190015) （Y16C190015）
江苏省海洋生物技术重点实验室开放基金(HS16001) （HS16001）
浙江新苗计划项目(2017R420004)资助[Supported by National Natural Science Foundation of China(31772846) （2017R420004）
Zhejiang Provincial Natural Science Foundation(Y16C190015) （Y16C190015）
Open-end Funds of Jiangsu Key Laboratory of Marine Biotechnology(HS16001) （HS16001）
Zhejiang Xinmiao Talents Program(2017R420004)] （2017R420004）
