水生生物学报2018，Vol.42Issue(3)：494-502,9.DOI:10.7541/2018.062
扇贝核糖体ITS1和5SrDNA序列的遗传变异及亲缘关系分析
GENETIC VARIATION AND RELATIONSHIP ANALYSIS OF SCALLOPS BASED ON THE SEQUENCES OF ITS1 AND 5S rDNA
摘要
Abstract
Rock scallop Crassadoma gigantea was introduced to China in 2012,and was successfully bred in 2015.It is in the stage of popularizing culture now. To understand the genetic relationships and the genetic variations among C. gigantea,Argopecten irradians,Chlamys farreri,and Patinopecten yessoensis,the whole sequences of ITS1 and 5S rDNA were sequenced after the amplification by PCR. The sequence characterization, genetic diversity, genetic dis-tance,and phylogeny were determined by using a suite of biology softwares.Based on the sequences of ITS1,the num-bers of haplotype and polymorphic sites, haplotype diversities, nucleotide diversities, and average nucleotide diffe-rences were varied from 2 to 6, 1 to 12, 0.425 to 0.800, 0.00081 to 0.00486 and 0.366 to 2.44, respectively. In addition, based on the sequences of 5S rDNA, their same genetic variation parameters varied from 2 to 5, 1 to 14, 0.533 to 0.841, 0.00108 to 0.1058 and 0.533 to 5.726, respectively. The result showed that all these four species had a low diversity level(π<0.01).Based on the sequences of ITS1 and 5S rDNA,the genetic distance were varied from 0.040 to 0.124,and 0.043 to 0.457,respectively,which demonstrated that C.gigantea appeared to be closely-related to P.yessoensis. Meanwhile,phylogeny trees of the genetic distances confirmed the genetic relatedness between C.gigantea and P.yes-soensis.This work would provide basic information for future studies on germplasm resources management,as well as on the genetics and breeding strategies of C.gigantea.关键词
ITS1/5SrDNA/岩扇贝(Crassadomagigantea)/遗传变异/亲缘关系Key words
ITS1/5S rDNA/Rock scallop(Crassadoma gigantea)/Genetic variation/Genetic relationship分类
生物科学引用本文复制引用
廖德杰,曹善茂,童金苟,周颖,俞小牧,刘阳,王潇..扇贝核糖体ITS1和5SrDNA序列的遗传变异及亲缘关系分析[J].水生生物学报,2018,42(3):494-502,9.基金项目
辽宁省海洋与渔业厅项目(201214) （201214）
大连市海洋与渔业局项目(201211) （201211）
淡水生态与生物技术国家重点实验室项目(2016FBZ05)资助[Supported by the Department Project of Liaoning Marine and Fisheries(201214) （2016FBZ05）
Department Project of Dalian Marine and Fisheries(201211) （201211）
Grant from the State Key Laboratory of Freshwater Ecology and Biotechnology of China(2016FBZ05)] （2016FBZ05）
