水生生物学报2018，Vol.42Issue(3)：503-511,9.DOI:10.7541/2018.063
中国米虾bursicon基因的功能及作用机制
THE FUNCTION AND MECHANISM OF BURSICON GENE IN CARIDINA
摘要
Abstract
To study the function of bursicon gene in Caridina during molting and cuticle formation,its open reading frame(ORF)containing two subunits genes were amplified by PCR respectively.The ORF of bursicon-α is 441 bp in length,encoding a protein with 146 amino acid residues.The ORF of bursicon-β is 411 bp in length,encoding a pro-tein with 136 amino acid residues. The dynamic changes in the expression of both bursicon subunit genes during the molt cycle in Caridina have been analyzed by the real-time quantitative PCR.Relative expression of both bursicon-α and bursicon-β had different levels in different stages of the molt cycle,which increased through premolt stage(D stage) and reached their peak level at the D3stage. The mRNA accumulation decreased to its lowest level at the molt stage(E stage).RNA interference-mediated knockdown of bursicon-α and bursicon-β retard the molting process and ecdysis behavior of Caridina.The cuticle of dsRNA-treated Caridina was thinner than that in the control group.These findings demonstrate that bursicon is involved in the thickening and hardening of cuticle in newly formed exoskeleton. Moreover, bursicon plays an important role in molt cycle.关键词
米虾/鞣化激素/表皮鞣化/蜕皮周期/实时荧光定量PCR/RNA干扰Key words
Caridina/Bursicon/Cuticular tanning/Molt cycle/Real-time quantitative PCR/RNA interference分类
生物科学引用本文复制引用
任丽琦,翁洁羊,王鑫,孙金生,李冉..中国米虾bursicon基因的功能及作用机制[J].水生生物学报,2018,42(3):503-511,9.基金项目
国家自然科学基金青年基金(31302168) （31302168）
天津市应用基础与前沿技术研究计划(14JCYBJC30700) （14JCYBJC30700）
天津师范大学博士基金(52XB1303)资助[Supported by the National Natural Science Foundation for Young Scientists of China(31302168) （52XB1303）
Tianjin Research Program of Application Foundation and Advanced Technology(14JCYBJC30700) （14JCYBJC30700）
the Ph.D.Program Foundation of Tianjin Normal University(52XB1303)] （52XB1303）
