水生生物学报2018，Vol.42Issue(3)：517-524,8.DOI:10.7541/2018.065
外源性脂肪酸对罗非鱼脂肪细胞增殖与分化的影响
EFFECTS OF EXOGENOUS FATTY ACID ON THE PROLIFERATION AND DIFFERENTIATION OF NILE TILAPIA PREADIPOCYTES
摘要
Abstract
To investigate the effects of fatty acids on the proliferation and differentiation of preadipocytes from Nile tilapia(Oreochromis niloticus),100 μmol/L Palmitic acid(PA),Oleic acid(OA),Linoleic acid(LA)and α-Linole-nic acid (LNA) were used. SRB (Sulforhodamine B) staining and oil red O staining were used to detect cell prolifera-tion and preadipocyte differentiation. The expression level of genes related to proliferation and differentiation was de-tected by Real-time qPCR. The results showed that exogenous fatty acids promoted the preadipocyte proliferation in 8 days,and significantly increased the expression level of proliferation related genes(c-fos and c-myc),lipolysis related genes(ATGL)and adipogenesis related genes(PPAR,CD36 and FAS)(P<0.05).In addition,fatty acid inhibited preadipocyte differentiation into adipocyte by decreasing lipid droplets area and increasing the lipid droplets number. During differentiation,expression level of β-oxidation related gene(CPT-1a)significantly increased(P<0.05),while the expression level of lipolysis related genes(ATG)and adipogenesis related genes(PPARγ and FAS)declined.Our results showed that exogenous fatty acid promoted the proliferation and inhibited preadipocyte differentiation. In the process of proliferation, excess fatty acids stored in the preadipocyte that could be metabolized by lipolysis and β-oxida-tion during adipogenesis to help cells adapt to high concentrations of fatty acids in the environment. While during the differentiation, the exogenous fatty acids can inhibit adipogenesis and lipolysis of adipocyte and promote β-oxidation to reduce adipocyte differentiation.关键词
罗非鱼/脂肪细胞/脂肪酸/增殖/分化Key words
Tilapia/Adipocyte/Fatty acid/Proliferation/Differentiation分类
农业科技引用本文复制引用
王雅文,乔芳,张美玲,贾永义,杜震宇..外源性脂肪酸对罗非鱼脂肪细胞增殖与分化的影响[J].水生生物学报,2018,42(3):517-524,8.基金项目
农业部淡水渔业健康养殖重点实验室、浙江省鱼类健康与营养重点实验室和浙江省淡水水产研究所开放课题(ZJK2014004) （ZJK2014004）
国家重大基础研究项目(2014CB138600) （2014CB138600）
国家自然科学基金(31472290)资助[Supported by Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture,Key Laboratory of Fish Health and Nutrition of Zhejiang Province,Zhejiang Institute of Freshwater Fisheries(ZJK2014004),the National Basic Research Program of China(2014CB138603),National Natural Science Foundation of China(31472290)] （31472290）
