水生生物学报2018，Vol.42Issue(3)：599-605,7.DOI:10.7541/2018.075
美洲鲥雄性生殖细胞冷冻保存及移植
STUDIES ON CRYOPRESERVATION AND TRANSPLANTATION OF THE MALE GERM CELL IN AMERICAN SHAD(ALOSA SAPIDISSIMA)
摘要
Abstract
Germ cells, different from somatic cells, carry the hereditary information and can transmit it to next genera-tion. The germ cells cryopreservation and culture are the key techniques for cell engineer breeding and genetic resource preservation. This study investigated the effects of cryopreservation of the testicular blocks or dissociated cells on the viability of male germ cells of American shad after 250 days using Hochest33342/Propidium Iodide (PI) staining. The results showed that the overall viability of germ cells from the testis blocks cryopreservation was higher than that of dissociated cells cryopreservation, suggesting that the tissue frozen could efficiently cryopreserve the germ cells at dif-ferent stages in American shad. Moreover, the thawed germ cells of American shad were stained with PKH26 before transplantation into newly-hatched zebrafish fries (1 days post hatching). At the 5thday post transplantation, the donor cells were still detected in some recipients and some donor cells were co-localized with the endogenous PGCs of reci-pient, implying that the germ cells of American shad after a long-term cryopreservation still possess the properties of germ cells and could integrate to the genital ridge of zebrafish host. The findings of this study provide technological and theoretical basis for further investigations on germ cell development, culture and genetic resource preservation in American shad or other anadromous fish in wild field.关键词
生殖细胞/冷冻保存/细胞移植/精巢/美洲鲥Key words
Germ cells/Cryopreservation/Cell transplantation/Testis/American shad分类
农业科技引用本文复制引用
吴栩灵,洪孝友,李凯彬,朱新平,徐红艳..美洲鲥雄性生殖细胞冷冻保存及移植[J].水生生物学报,2018,42(3):599-605,7.基金项目
广东省科技计划项目(2014A020208037和2016A050502029)资助[Supported by Science and Technology Projects of Guangdong Province(2014A020208037,2016A050502029)] （2014A020208037和2016A050502029）
