水生生物学报2018，Vol.42Issue(3)：635-640,6.DOI:10.7541/2018.079
集胞藻PCC6803高产精氨酸藻株的紫外诱变选育
CREATION OF HIGH ARGININE PRODUCING STRAIN OF SYNECHOCYSTIS SP. PCC 6803 BY UV MUTAGENESIS
摘要
Abstract
Arginine has been widely used in medicine and food industry.Synechocystis sp.PCC 6803,a unicellular cya-nobacterium, synthesize amino acids and other compounds using nitrate or nitrite as the nitrogen source. In flue gases released by industrial production, NOx(nitrogen oxides) can react with water to form nitrate and nitrite, which can be utilized by cyanobacteria.Hence,creating high arginine producing strain of Synechocystis sp.PCC 6803 has a strong application prospects, in denitrification of industrial flue gases. This study reported the successful creation of a high ar-ginine producing strain#13807-111-55 of Synechocystis sp.PCC 6803 by 0.8 g/L D-arginine and 0.2 g/L 6-azaruacil resistant mutants with UV mutagenesis. Compared with the parent strain #13807, extracellular arginine production per OD730of #13807-111-55 increased 62.3-fold, reaching (0.76±0.1) mg/(L·OD730). Total arginine production per OD730 of #13807-111-55 increased about 6.0-fold, reaching (0.82±0.08) mg/(L·OD730). Extracellular arginine production per OD730of the mutant was significantly higher than that of intracellular, showing that the strain was the arginine-secret-ing mutant with strong production potential.关键词
紫外诱变/精氨酸/集胞藻PCC6803Key words
UV mutagenesis/Arginine/Synechocystis sp.PCC 6803分类
农业科技引用本文复制引用
戢水玲,高宏..集胞藻PCC6803高产精氨酸藻株的紫外诱变选育[J].水生生物学报,2018,42(3):635-640,6.基金项目
中国科学院科技服务网络计划(STS)项目资助[Supported by the STS Project of Chinese Academy of Sciences] （STS）
