水生生物学报2018，Vol.42Issue(3)：655-662,8.DOI:10.7541/2018.081
裸藻遗传转化技术的研究进展
RESEARCH PROGRESS ON GENETIC TRANSFORMATION TECHNOLOGY OF EUGLENA
摘要
Abstract
Euglena have important scientific value for studying biological evolution and chloroplast endosymbiosis and provide high value-added dietary supplements as well as high-quality feedstock for biofuel production. Studies of Euglena have been mainly explored by physiological and biochemical methods.With almost completed Euglena ge-nome, we employed diverse OMIC techniques such as single cell RNA-seq, transcriptomic, proteomic, methylomic, and metabolomics to study the chloroplast development and the communication between chloroplast and nucleus. We hooked up some interesting genes and microRNA targets. Little progress has been achieved in genetic engineering of Euglena due to lack of efficient techniques for genetic operation.In here,we reviewed the methods in genetic trans-formation of Euglena,such as antibiotic biomarkers,biolistic,electroporation,glass beads and Agrobacterium trans-formation techniques, RNAi, potential CRISPR/cas9, and cpf1 genome editing, to provide a reference for functional ge-nomics and biotechnology of Euglena.关键词
裸藻/叶绿体/基因组/遗传转化/RNA干扰Key words
Euglena/Chloroplast/Genome/Genetic transformation/RNAi分类
生物科学引用本文复制引用
邵青,蒋永光,雷安平,胡章立,王江新..裸藻遗传转化技术的研究进展[J].水生生物学报,2018,42(3):655-662,8.基金项目
国家自然科学基金(31500071和31670116) （31500071和31670116）
中国博士后科学基金特别资助项目(2016T90801)资助[Supported by the National Natural Science Foundation of China(31500071 and 31670116) （2016T90801）
The Postdoctoral Science Fund Special Funding Project of China(2016T90801)] （2016T90801）
