生态学报2018，Vol.38Issue(8)：2816-2828,13.DOI:10.5846/stxb201705210939
炉渣与生物炭施加对稻田土壤产甲烷菌群落结构的影响
Effects of slag and biochar amendments on methanogenic community structures in paddy fields
摘要
Abstract
To understand the effects of waste amendments on microbiological mechanisms of methane emissions in paddy fields,the effects of single and mixed slag and biochar amendments on soil physicochemical properties were investigated in the elongation stage of both early and late paddy fields.Moreover,the diversity of methanogens and their community structures under different waste amendments were also investigated using PCR-RFLP and sequencing.The results showed that during the elongation stage of early paddy fields,both soil salinity and pH were significantly increased in mixed amendments.During the elongation stage of late paddy fields,soil salinity was significantly increased in mixed amendments,while soil pH was significantly increased in slag and mixed amendments.Shannon index (H') and Simpson diversity index (D) showed that the diversity of methanogens in soil treated with slag,biochar,and mixed amendments was higher than that of the control.Phylogenetic analysis showed that methanogeni communities in the paddy fields could be subdivided into six groups:Methanobacteriales,Methanomicrobiales,Methanosarcinales,Methanococcales,Methanocellales,and Methanomassiliicoccales.The most dominant methanogenic group was Methanomicrobiales.Comparison with the control,the relative abundance of Methanomassiliicoccus was lowered in the three types of treatments of the early paddy fields,and the relative abundance of Methanosarcina was lowered in biochar amendment.The relative abundance of Methanomassiliicoccus and Methanosarcina may have contributed to decreased CH4 emissions.关键词
产甲烷菌/群落结构/废弃物/PCR-RFLP/稻田Key words
methanogens/community structures/waste/PCR-RFLP/paddy fields引用本文复制引用
王妙莹,许旭萍,王维奇,王广磊,苏程举..炉渣与生物炭施加对稻田土壤产甲烷菌群落结构的影响[J].生态学报,2018,38(8):2816-2828,13.基金项目
国家自然科学基金项目(41571287,31000209) （41571287,31000209）
福建省科技厅重点项目(2014Y0054,2014R1034-3) （2014Y0054,2014R1034-3）
福建省自然科学基金项目(2014J01119) （2014J01119）
福建省公益类研究所专项(2018R1101006-1) （2018R1101006-1）
福建省高校杰出青年科研人才培育计划 （）
