生物骨科材料与临床研究2018，Vol.15Issue(2)：9-13,后插2-后插3,7.DOI:10.3969/j.issn.1672-5972.2018.02.003
含纳米银颗粒的β-磷酸三钙复合支架的抗菌性和促成骨性的研究
Silver nanoparticle-incorporated β-tricalcium phosphate scaffold with antibacterial and osteogenic activity
摘要
Abstract
Objective To investigate the antibacterial and osteogenic activity of 5％ (mass fration) elemental silver nanoparticle-incorporated β-tricalcium phosphate scaffold (β-TCP-Ag) as a new idea for treating osteomyelitis with large area of bone defect.Methods In vitro:the cytotoxicity of the β-TCP-Ag was characterized by Cell Counting Kit-8 assay in comparison to pure β-tricalcium phosphate scaffold.In vivo:28 New Zealand rabbits underwent osteomyelitis of the left femoral condyle with staphylococcus aureus infection for 4 weeks,then were implanted scaffold (β-TCP-Ag or β-TCP).After 4 weeks' raising,the rabbits were sacrificed to collected the bone tissue around the scaffold,then the relative number of staphylococcus aureus was detected by spread plate method.After 12 weeks' raising,the rabbits were sacrificed and microcomputed tomography (Micro-CT) was used to observe the bone healing around the scaffold.Results The result of cytotoxicity test show that there is no significant difference of the relative cell number between the β-TCP-Ag group and the control group.The result of spread plate method show that the relative number of staphylococcus aureus in the β-TCP-Ag group is remarkable higher than that of the control group.The Micro-CT analysis show that the bone volume/total volume (BV/TV) of β-TCP-Ag group is significant higher than that of the control group.Conclusion The above results indicates that this potential biomaterial for treating osteomyelitis with bone defect has good biocompatibility,antibacterial activity and osteogenic activity.This study demonstrates that the β-TCP-Ag with a combination of antibacterial and osteogenic activity can be achieved for the reconstruction of bone defects while treating infections.关键词
纳米银颗粒/β-磷酸三钙支架/骨髓炎/抗菌性/成骨活性Key words
Silver nanoparticle/β-tricalcium phosphate scaffold/Osteomyelitis/Antibacterial activity/Osteogenic activity分类
医药卫生引用本文复制引用
管捷,程坚,谢幼专,卢建熙..含纳米银颗粒的β-磷酸三钙复合支架的抗菌性和促成骨性的研究[J].生物骨科材料与临床研究,2018,15(2):9-13,后插2-后插3,7.基金项目
1国家自然科学基金(81071453) （81071453）
上海市科委基金(1441901700) （1441901700）
