山西医科大学学报2018，Vol.49Issue(4)：375-382,8.DOI:10.13753/j.issn.1007-6611.2018.04.010
UPLC-MS分析柴胡、白芍水煎液的化学成分及其对逍遥散的贡献
Analysis on chemical constituents in Bupleuri Radix and Paeoniae Radix Alba by UPLC-MS and their contribution to Xiaoyao powder
摘要
Abstract
Objective To investigate the chemical constituents in extract of Bupleuri Radix and Paeoniae Radix Alba using UPLC-MS and confirm their contributions to Xiaoyaosan.Methods These compounds were analyzed with a Waters Acquity UPLC HSS T3 column (2.1 mmx 100 mm,1.8 μm) by gradient elution of acetonitrile(A) and 0.1％ H2O-formic acid(B).The flow rate was 0.3 ml/min,and the sample volume was 1 μl.The mass spectrometry information and the related literature were used to analyze the main chemical constituents of Bupleuri Radix and Paeoniae Radix Alba.Results Eighty-six chemical constituents were found in Xiaoyao powder decoction by UPLC-MS.Among them,44 were identified,of which,13 were from Bupleuri Radix and 31were from Paeoniae Radix A1ba.Conclusion UPLC-MS could quickly,accurately and comprehensively analyze the chemical constituents of Xiaoyao powder,Bupleuri Radix and Paeoniae Radix Alba,which could provide a scientific evidence for the development of new drugs and the therapeutic material basis.关键词
UPLC-MS/逍遥散/柴胡/白芍/化学成分Key words
UPLC-MS/Xiaoyaosan/Bupleuri Radix/Paeoniae Radix Alba/chemical constituents分类
医药卫生引用本文复制引用
贺晶,高晓霞,田俊生,秦雪梅,杜冠华,周玉枝..UPLC-MS分析柴胡、白芍水煎液的化学成分及其对逍遥散的贡献[J].山西医科大学学报,2018,49(4):375-382,8.基金项目
国家自然科学基金面上项目(81673572) （81673572）
山西省应用基础研究项目(201601D021164) （201601D021164）
山西省高校科技创新项目(2016120) （2016120）
