生殖医学杂志2018，Vol.27Issue(5)：422-425,4.DOI:10.3969/j.issn.1004-3845.2018.05.006
不同生理阶段小鼠雌激素受体水平及IL-13、TNF-α含量的变化
Estrogen receptor level and content change of IL-13 & TNF-α in different physiological stages in mice
摘要
Abstract
Objective:To observe estrogen receptor level and the content change of interleukins 13(IL-13) and tumor necrosis factor alpha(TNF-α) in different physiological stages of change in mice,and explore the effect of estrogen on immunity.Methods:Sixty clean grade healthy female C57BL/6 mice were selected.Among them,40 mice with two month old were randomly divided into estrus group(control group) and pregnancy group,and 20 mice with ten month old were proposed to be the natural menopause group.PT-PCR technique was used to detect spleen IL-13,TNF-α,ERα and ERβ mRNA level,and ELISA was used to detect serum E2 level.The differences of the indicators were compared among the three groups.Results..Compared with control group,the serum E2 levels [(82.27±8.70pmol/L vs.55.23±5.11pmol/L)]and ERα mRNA level[(3.93±1.54) vs.(2.70±1.17)]were significantly increased in pregnancy mice(P＜0.05).The E2 1evel [(23.52±4.46)pmol/L vs.(55.23±5.11)pmol/L]and ERα mRNA level [(1.15±0.71) vs.(2.70±1.17)]were significantly decreased in natural menopause group(P＜0.05).The IL-13 and TNF-α mRNA levels in spleen of the pregnant mice were significantly decreased,while the IL-13 and TNF-α levels in spleen of the menopausal mice were significantly increased(P＜0.05).There was no significant difference in ERβ mRNA level among the three groups(P＞0.05).Conclusions:IL-13 and TNF-α expression levels in postmenopausal mice were significantly increased compared with the estrus mice,which may be associated with decreased levels of estrogen and ER alpha receptors in postmenopausal mice.关键词
小鼠/激素/受体水平/白细胞介素13/肿瘤坏死因子αKey words
Mouse/Hormone/Receptor level/Interleukins 13/Tumor necrosis factor alpha引用本文复制引用
唐飞,徐娟,邵明君..不同生理阶段小鼠雌激素受体水平及IL-13、TNF-α含量的变化[J].生殖医学杂志,2018,27(5):422-425,4.基金项目
浙江省自然科学基金(Y13H040019) （Y13H040019）
