生殖医学杂志2018，Vol.27Issue(5)：426-430,5.DOI:10.3969/j.issn.1004-3845.2018.05.007
肥胖对雄性大鼠生殖功能影响的机制研究
A research on mechanism of obesity on reproduction in male rat
摘要
Abstract
Objective:To investigate the effect of obesity on fertility in male rat,and preliminarily explore the mechanism of oxidative damage.Methods:The six-week-old SD male rats were randomly divided into the control(normal diet,n =15)and obese groups (high-fat diet,n =30).The body weight,organ indexes,serum metabolic parameters,reproductive hormones(GnRH,FSH,LH,total T,E2) and SHBG of two groups were assayed after feeding for 16 weeks.Indicators of reproductive function,including apoptosis of spermatogenic cells,oxidative stress,and sperm count and motility were determined.The differences between the two groups were compared.Results:Body weight and Lee index were significantly increased,and the level of leptin [(3.17±0.23)vs.(2.33±0.21) ng/ml],insulin [(28.99±2.43) vs.(20.62±1.72) μU/ml] and TG [(0.55±0.03) vs.(0.36±0.04) mmol/L] were significantly increased(P＜0.05),and GnRH [(36.46±1.95) vs.(45.51±2.75) pg/ml],LH [(3.06±0.37) vs.(4.67±0.70) U/L],T [(0.81±0.13) vs.(1.98±0.32) ng/ml],SHBG [(55.31±3.80) vs.(71.69±4.65) nmol/L] were significantly decreased(P＜0.05) in obese ratscompared with the control group.In addition,the apoptosis index [(4.26±0.35)％ vs.(3.09±0.28)％]of spermatogenic cells in testis was significantly increased in obese rats by TUNEL assay.The oxidativedamage with increased SOD and decreased MDA in obese rats was significantly different from that incontrol group(P＜0.05).Compared with the control group,sperm motility [(14.36±7.67) ％ vs.(36.40±9.17)％] was significantly decreased (P＜0.01),while the sperm concentration was not significantlydecreased(P＞0.05).Conclusions:Obesity induced by high-fat diet leads to decrease sperm motility in male rats,which maybe associated with metabolic and reproductive endocrine disorders,as well as apoptosis and oxidativedamage of testicular tissue.关键词
肥胖/生殖功能/代谢紊乱/生殖内分泌/氧化应激Key words
Obesity/Reproductive function/Metabolic disorder/Reproductive endocrine/Oxidative stress引用本文复制引用
郭颖,王晓尉,周芳,安琪,谷翊群..肥胖对雄性大鼠生殖功能影响的机制研究[J].生殖医学杂志,2018,27(5):426-430,5.基金项目
十二五国家科技支撑计划(2012BA132B03) （2012BA132B03）
