生殖医学杂志2018，Vol.27Issue(5)：431-436,6.DOI:10.3969/j.issn.1004-3845.2018.05.008
精子顶体相关基因SPACA7的转录活性研究
Study on transcription activity of sperm acrosome related gene SPACA7
摘要
Abstract
Objective:To investigate the promoter activity in the region from-2000 bp upstream to+ 84 bp downstream of the transcription initiation site of sperm acrosome related gene SPACA7.Methods:A total of 16 promoters of different lengths in the sequence of-2000 bp upstream and 84 bp downstream of the transcription start site of human SPACA7 gene were obtained by promoter truncation experiments.The 293T genomic DNAs of human kidney epithelial cells were used as a template for PCR.The different lengths of promoters were recombined into luciferase expression vector pGL3-basic and transfected cells.After 24 hours of transfection,the cells were collected and lysates were added,and then the cell lysates were collected.The luciferase activity was measured by using a multi-functional microplate reader.Results:The SPACA7 promoter from-2000bp to+84bp sequence was investigated,and 16 different length of the promoters::luciferase were constructed.Luciferase activity test results found that each promoter has certain activity;in which-1500bp to+84bp sequence promoter activity is the highest.Conclusions:It was initially proved that the sperm acrosome-related gene SPACA7 promoter has strong transcriptional activity in the-1500 bp to+84 bp region.关键词
启动子/荧光素酶/转录活性/精子顶体Key words
Promoter/Luciferase/Transcriptional activity/Sperm acrosome引用本文复制引用
张菁华,周永翠,唐爱发..精子顶体相关基因SPACA7的转录活性研究[J].生殖医学杂志,2018,27(5):431-436,6.基金项目
深圳市科技计划项目(JCYJ20170306091933286) （JCYJ20170306091933286）
深圳市卫生计生系统博士启动科研项目(SZBC2017009) （SZBC2017009）
