生殖医学杂志2018，Vol.27Issue(5)：460-464,5.DOI:10.3969/j.issn.1004-3845.2018.05.012
蜕膜CXCR6+/-单核-巨噬细胞在人早孕期母胎界面的表型差异
Phenotypic differences of decidual CXCR6+/-monocyte-macrophages in maternal-fetal interface during human early pregnancy
摘要
Abstract
Objective:To analyze the proportion and phenotypic differences of decidua CXCR6+ monocyte-macrophages in women with normal early pregnancy or unexplained spontaneous abortion.Methods:The decidual tissues were collected from normal early pregnancy women(n=40) and the patients with unexplained spontaneous abortion (n =20).The decidual immune cell populations were separated and the proportion of mononuclear macrophages was analyzed by flow cytometry in normal early pregnancy group and unexplained spontaneous abortion group.The differences in the expression of CXCR6+,CD80,and CD206 on the surface of decidual mononuclear macrophages(dMΦ) were compared between the two groups.Results:The proportion of CD14+ dMΦ in the decidual immune cell population (DIC) in the unexplained spontaneous abortion group was(9.9±1.63)％,and the proportion of CD14+ dMΦ in DIC in the normal early pregnancy group was(8.9±0.88)％.There was no significant difference between the two groups(P＞0.05).The expression rate of CXCR6+ in dMΦ in normal early pregnancy group [(40.0±1.90)％] was significantly higher than that in unexplained spontaneous abortion group [(31.22±2.38)％](P＜0.05).The expression of CD206 and CD80 in CXCR6+ dMΦ was significantly higher than that in CXCR6-dMΦ in normal early pregnancy group(P＜0.05).The expression of CD206 in CXCR6+ dMΦ was significantly higher than that in CXCR6-dMΦ in unexplained spontaneous abortion group(P＜0.01),but CD80 had no significant change(P＞0.05).Compared with normal early pregnancy group,the expression of CD206 in CXCR6+/-dMΦ was significantly decreased in the unexplained spontaneous abortion group (P＜0.05).The expression of CD80 in CXCR6-dMΦ was significantly increased(P＜0.05),and there was no significant difference in the expression of CXCR6+ dMΦ(P＞0.05).Conclusions:There was no significant change in the number of decidual mononuclear macrophages among women with unexplained spontaneous abortion.The expression of surface molecule CD206 of CXCR6+ dMΦ is significantly decreased and CD80 expression is significantly increased,which suggests that CXCR6+ dMΦ may be involved in pathogenesis in spontaneous abortion.关键词
母胎界面/单核巨噬细胞/自然流产/CXCR6Key words
Maternal-fetal interface/Monocyte-macrophage/Miscarriage pregnancy/CXCR6引用本文复制引用
刘倩倩,黄煜,颜莉莉,张鹏,张清宇,杜美蓉..蜕膜CXCR6+/-单核-巨噬细胞在人早孕期母胎界面的表型差异[J].生殖医学杂志,2018,27(5):460-464,5.基金项目
国家自然科学基金青年科学基金项目(30700763) （30700763）
山东省优秀中青年科学家科研奖励基金资助项目(BS2009SW002) （BS2009SW002）
山东省自然科学基金资助项目(ZR2013HM012) （ZR2013HM012）
国家自然科学基金青年科学基金项目(81502246) （81502246）
