天津中医药2018，Vol.35Issue(5)：344-348,5.DOI:10.11656/j.issn.1672-1519.2018.05.07
健脾利湿汤治疗轻中度溃疡性结肠炎的疗效及其对调节T细胞功能影响的机制研究
Efficacy and mechanism of Jianpi Lishi decoction in the treatment of patients with mild to moderate ulcerative colitis by regulating the function of Treg cells
摘要
Abstract
[Objective]In this study, the clinical efficacy of Jianpi Lishi decoction on mild to moderate ulcerative colitis (UC) and mechanisms associated with the function of Tregs were investigated. [Methods]Altogether 59 patients with mild to moderate UC were enrolled and randomly allocated to the control and Jianpi Lishi groups.The control group received salbutamol enteric-coated tablets,and the Jianpi Lishi group was given Jianpi Lishi decoction orally.The treatment process would be lasted for 3 months.The main symptom scores,curative effect index and the quality of life of patients were observed before and after treatment.The concentrations of IL-1β and TGF-β1 in peripheral blood were measured by enzyme-linked immunosorbent assay(ELISA)and the mRNA levels of Foxp3,perforin and granzyme B were determined by RT-qPCR. [Results]After treatment,the above symptoms were improved significantly,and the effect of"Jianpi Lishi"group was much better than that of the control group (P<0.05). After 3 months of treatment, the quality of life in gastrointestinal symptoms,systemic symptoms,affective ability and social ability of the two groups were significantly higher than those before treatment (P<0.05),and the quality of life was significantly better in the Jianpi Lishi group when compared with the control group (P<0.05).Compared with normal subjects,the levels of IL-1β in peripheral blood were higher in the two groups before treatment,while the level of TGF-β was lower;the mRNA level of Foxp3 was lower,and the mRNA levels of perforin and granzyme B were higher(P<0.05 or P<0.01).After treatment,the levels of above-mentioned cytokines and genes were changed to the opposite direction with significant differences (P<0.05 or P<0.01),and the improvement effect of Jianpi Lishi group was more obvious than the control group (P<0.05). [Conclusion]Jianpi Lishi decoction can enhance the immunosuppressive function by regulating the number and function of Tregs,and inhibit the killing effect of perforin/granzyme B on target cells,so as to reduce the inflammatory reaction of intestinal tract.关键词
溃疡性结肠炎/调节T细胞/穿孔素/颗粒酶BKey words
ulcerative colitis/regulatory T cells/perforin/granzyme B分类
医药卫生引用本文复制引用
郝莉莉,刘小溪..健脾利湿汤治疗轻中度溃疡性结肠炎的疗效及其对调节T细胞功能影响的机制研究[J].天津中医药,2018,35(5):344-348,5.基金项目
天津市卫生局基金项目(2015107). （2015107）
