天津中医药2018，Vol.35Issue(5)：349-354,6.DOI:10.11656/j.issn.1672-1519.2018.05.08
过敏性鼻炎患者肺虚感寒证和肾阳亏虚证血清代谢组学研究
Study on serum metabolomics of lung deficiency and cold syndrome and kidney-yang deficiency syndrome in patients with allergic rhinitis
摘要
Abstract
[Objective]Based on GC/MS metabolomics techniques,endogenoussmall molecule metabolitesof serum onlung deficiency and cold syndrome and kidney-yang deficiency syndrome in patients with allergic rhinitiswere analyzed, and thereby providing scientific evidence for syndrome typing of them. [Methods]Small molecule metabolites in the serum of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome as well as normal control group were detected with GC/MS.PCA and PLS-DA were analyzed using SIMCA-P software,differences of small molecule metabolites inserum among the groups was studied. [Results]Metabolic profile appeared good separation trend between allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome, three groups were distinguished clearly. The 23 statistically significant differences metabolites were identified using commercially available metabolite libraries (such as the Wiley and NIST mass pools)and the standard metabolite library established in our laboratory. Four differentially identified metabolites were screened from serum of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndrome included alanine,tetradecanoic acid,hexadecanoic acid,9,12-octadecadienoic acid,9-(Z)-O-octadecenoic acid,octadecanoic acid. [Conclusion]The metabolic differences of allergic rhinitis with lung deficiency and cold syndrome and kidney-yang deficiency syndromemainly manifested in amino acid metabolism and nucleotide metabolism,and based on GC/MS metabolomics techniquesmay be used in classified study of Chinese medical syndrome typing.关键词
代谢组学/肺虚感寒证/肾阳亏虚证/中医证型/过敏性鼻炎Key words
metabonomics/lung deficiency and cold syndrome/kidney-yang deficiency syndrome/traditional Chinese medicine syndrome/allergic rhinitis分类
医药卫生引用本文复制引用
梁薇,杨艳,李晓燕,赵创,苟小军..过敏性鼻炎患者肺虚感寒证和肾阳亏虚证血清代谢组学研究[J].天津中医药,2018,35(5):349-354,6.基金项目
国家自然科学基金项目(81473475) （81473475）
宝山区科技创新专项基金项目(13-E-18) （13-E-18）
宝山卫生青年医学人才培养计划(bswsyq-2017-A8). （bswsyq-2017-A8）
