天津中医药2018，Vol.35Issue(5)：376-380,5.DOI:10.11656/j.issn.1672-1519.2018.05.14
豨红通络口服液改善小鼠炎性和神经病理性疼痛的作用研究
Inflammatory and neuropathic pain in mice reversed by Xihong Tongluo oral liquid
摘要
Abstract
[Objective]To investigate the effect of anti-inflammatory and neurophatic pain of Xihong Tongluo oral liquid.[Methods]A dimethyl benzene-induced mouse auris swelling model was established to observe anti-inflammatory effects.The inflammatory pain was induced by a subcutaneous injection of 50 μL CFA into the bottom of left hind paws of mice.The response to writhing and stretching reaction induced by acetic acid (HAc,0.6%,50 μL)injection into a peritoneal cavity of mice was evaluated.A central poststroke pain (CPSP)model was used with a single collagenase (type IV;0.25 μL,0.025 U)injection into the left ventral posterolateral nucleus of the thalamus.Xihong Tongluo oral liquid was administrated to mice via intragastric administration and saline was administrated as a control. Then at corresponding time points (1/48 d, 1 d, 3 d, 5 d), inflammatory swelling, pain related-behaviors and histopathology were conducted and evaluated blindly.[Results]The experimental results of ear swelling showed that Xihong Tongluo oral liquid(low-dosage:20 L/g;high-dosage:100 L/g)exerted positive regulation in a time-dependent manner,in a comparison with model group.On the fifth day, ear edema of mice in the high-dose group was significantly alleviated compared with other groups. The edema of hind paw in CFA-inflamed mice was obvious on the first day. While significant reductions were seen in the group intragastric administrate with Xihong Tongluo oral liquid,while the high-dosage group showed the best anti-inflammatory effect;HE staining revealed that gavage treatment of Xihong Tongluo oral liquid can significantly alleviated the CFA-induced inflammatory infiltration.With gavage treatment,the number of writhing in HAc groups was significantly reduced, and high-dosage group showed significant analgesic effect on the 5th day. Nissl staining showed that CPSP weakened Nissl body staining and increased the amount of hypertrophic glial cells. [Conclusion] Those symptoms were improved after gavage treatment of Xihong Tongluo oral liquid. Xihong Tongluo oral liquid can relieve inflammatory swelling and pain response.While the injured neuron and hypertrophic glial cells induced by CPSP were improved,Xihong Tongluo oral liquid exerted greater anti-inflammatory and analgesic effects on central neuropathic pain.关键词
豨红通络口服液/炎性肿胀/炎性疼痛/脑卒中/神经病理性疼痛Key words
Xihong Tongluo oral liquid/inflammatory swelling/inflammatory pain/stroke/neuropathic pain分类
医药卫生引用本文复制引用
张建,田贵华,黄凤珍,张炜,李小军,李玉桑,唐潇旖,胡烨胤,商洪才,唐和斌..豨红通络口服液改善小鼠炎性和神经病理性疼痛的作用研究[J].天津中医药,2018,35(5):376-380,5.基金项目
国家自然科学基金项目(81373842,81674050,81403468). （81373842,81674050,81403468）
