天津中医药大学学报2018，Vol.37Issue(2)：149-154,6.DOI:10.11656/j.issn.1673-9043.2018.02.15
复方车前凝胶贴膏的制备及初步评价
Preparation and preliminary evaluation of compound plantaginis semen gel paste
摘要
Abstract
[Objective]The aim of this study is to prepare compound Plantaginis semen Extractum(CPE)as an easy-to-use Compound Plantaginis semen Gel Paste(CPGP). [Methods] Plackett-Burman design combined with Box-Behnken esponse surface methodology was used to optimize the preparation formulation with initial bonding strength, holding force,peel strength of 180°as the evaluation index.Modified Franz diffusion cell was used to study in vitro release behavior of CPGP.A preliminary evaluation of the efficacy of CPE and CPGP on the H22 hepatoma ascites model in mice was carried out. [Results]The optimized matrix formulation of CPGP is NP700,PVPK90,glycerol, aluminium glycinate,tartaric acid,distilled water(8:5.38:32.5:0.5:0.5:44.62).The in vitro release rate of geniposide in the CPGP was(81.23±1.52)% at 24 h.The results showed that the CPGP formulation was reasonable.The viscosity of gel paste prepared by the formulation was suitable. In animal experiment, compared with the model group, the volume of ascites mice grouped in CPGP and CPE was very significantly decreased(P<0.01)and the VEGF content in ascites was significantly decreased(P<0.05),also the destruction of immune function was significantly improved (P<0.05) after the treatment for 7 days. Meanwhile the gel paste group and extract group showed no significant difference. [Conclusion]Therefore,the research showed a reasonable CPGP prescription.The release rate of the target component geniposide was in accord with the first order kinetic equation. CPGP effectively reduced the amount of ascites in mice.关键词
Plackett-Burman/Box-Behnken/凝胶贴膏/体外释放/H22肝癌腹水模型Key words
Plackett-Burman/Box-Behnken/Gel Paste/in vitro release/H22 hepatoma ascites model分类
医药卫生引用本文复制引用
夏珍珍,陈柽,李冰韶,王永洁,谢远平,林红梅,吴清..复方车前凝胶贴膏的制备及初步评价[J].天津中医药大学学报,2018,37(2):149-154,6.基金项目
国家自然科学基金资助项目(81473365). （81473365）
