西北师范大学学报（自然科学版）2018，Vol.54Issue(3)：57-64,8.DOI:10.16783/j.cnki.nwnuz.2018.03.011
原花青素对鱼藤酮诱导PC12细胞损伤的保护作用及机制研究
Protective effect and mechanism of procyanidin on rotenone induced PC12 cell damage
摘要
Abstract
Parkinson's disease(PD)is a common neurodegenerative disorder in elderly population.On account of increasing the incidence rate,its etiology and prevention strategy must be clarified.In this study,dopaminergic neuronal cell model PC12 were treated with rotenone(Rot),and the inhibitory effect of grape seeds procyanidins(GSP)on Rot cytotoxicity was investigated.MTT assay showed that GSP can attenuate the inhibitory effect of Rot on cell growth in dose-and time-dependent pattern.Under inverted microscope,Giemsa and Hoechst 33342 stained cells showed that Rot can damage cells significantly on cell morphology.Cell is changed from flat polygons to shrinkage,neurites is retracted,nuclear chromatin is agglutinated,and some nuclei are fragmented, showing a certain apoptosis characteristics. GSP pre-treatment has obvious protective activity on the changes of cell and nuclear morphology.After staining with DCFH-DA and Rhodamine 123,flow cytometry analysis and fluorescence microscopy showed that after GSP treatment,the content of reactive oxygen species(ROS)in cells treated with Rot is decreased significantly and the mitochondria membrane potential(M M Ps)are increased.Annexin V/PI double staining test exhibited that GSP is reduced apoptosis by Rot.These results indicated that GSP can protect cells,inhibit the apoptosis induced by Rot in PC12 cell through down-regulating the production of ROS and restoring mitochondrial membrane.GSP may play a role in the prevention and treatment of Parkinson's disease.关键词
帕金森症/鱼藤酮/原花青素/凋亡/PC12细胞Key words
Parkinson disease/proanthocyanidin/rotenone/apoptosis/PC12 cell分类
医药卫生引用本文复制引用
刘国安,吴亚楠,董欢,冉苗苗,孟凡雷..原花青素对鱼藤酮诱导PC12细胞损伤的保护作用及机制研究[J].西北师范大学学报（自然科学版）,2018,54(3):57-64,8.基金项目
甘肃省自然科学基金资助项目(17JR5RA081) （17JR5RA081）
