中国组织工程研究2018，Vol.22Issue(12)：1817-1822,6.DOI:10.3969/j.issn.2095-4344.0802
基于内质网应激研究蛇床子素对成骨细胞增殖与分化的影响
Osthol effects on the proliferation and differentiation of osteoblasts based on endoplasmic reticulum stress
摘要
Abstract
BACKGROUND: Osthol has been reported to promote osteogenesis by increasing osteoblast proliferation, but the anti-osteoporosis mechanism underlying osthol is poorly understood.OBJECTIVE:To observe the effect of osthol on the proliferation and differentiation of rat osteoblasts in vitro and to explore its mechanism of anti-osteoporosis effect. METHODS: Rat osteoblasts were isolated by secondary enzyme digestion and identified by alkaline phosphatase staining and mineralized nodule staining. There were five groups: blank control, solvent control, β-estradiol as well as low-, medium- and high-dose osthol groups. Cell proliferation was detected by cell counting kit-8 assay, and cell differentiation was evaluated by detection of alkaline phosphatase and mineralized nodule staining. The expression levels of GRP78, PDI and CHOP were detected by western blot assay. RESULTS AND CONCLUSION: Osthol at the concentrations of 1x10-4and 1x10-5mol/L could inhibit osteoblast proliferation. 1x10-4mol/L osthol could increase the activity of alkaline phosphatase in osteoblasts and enhance osteoblastic mineralization. Meanwhile, 1x10-4mol/L osthol was able to down-regulate the expression level of GRP78 and up-regulate the expression levels of PDI and CHOP. To conclude, osthol can promote osteoblast differentiation and inhibit osteoblast proliferation probably by endoplasmic reticulum stress.关键词
蛇床子素/成骨细胞/内质网应激/GRP78/PDI/CHOP/组织构建分类
医药卫生引用本文复制引用
郑苏阳,马勇,郭杨,王礼宁,潘娅岚..基于内质网应激研究蛇床子素对成骨细胞增殖与分化的影响[J].中国组织工程研究,2018,22(12):1817-1822,6.基金项目
国家自然科学基金面上项目(81473692) （81473692）
江苏省高校优势学科南京中医药大学护理学(苏政办发2014(37)号)资助项目 （苏政办发2014(37）
江苏省中医药局科技项目(YB2015135)the National Natural Science Foundation of China,No.81473692 （YB2015135）
Superior Discipline Construction Project for the Nursing of Nanjing University of Chinese Medicine,No.2014(37) （37）
the Scientific and Technological Project of Jiangsu Provincial Administration of TCM,No.YB2015135 （）
