中国组织工程研究2018，Vol.22Issue(12)：1866-1871,6.DOI:10.3969/j.issn.2095-4344.0828
独活寄生汤干预食蟹猴自发性膝骨关节炎模型炎症的作用
Effect of Duhuojisheng Decoction on primary knee osteoarthritis in macaca fascicularis
摘要
Abstract
BACKGROUND:Meta-analysis results have shown that Duhuojisheng Decoction can improve the symptoms and relieve the pain of patients with knee osteoarthritis, but the mechanism and pathway are not very clear.OBJECTIVE:To further verify the curative effect of Duhuojisheng Decoction for primary knee osteoarthritis in macaca fascicularis. METHODS: Under natural conditions, 6 clean young macaca fascicularis (aged 3-5 years, normal group) and 6 elderly macaca fascicularis (aged more than 20 years, model group) were selected from 24 macaca fascicularis. The models of primary knee osteoarthritis were established in the model group,then aged animals were randomly divided into control and intervention(given the treatment of Duhuojisheng Decoction) groups. The number of whole blood leukocytes, C-reactive protein, erythrocyte sedimentation rate, properties of the joint fluid and expression level of tumor necrosis factor-α were compared between the control and intervention groups. RESULTS AND CONCLUSION: The aged macaca fascicularis was characterized by a typical osteoarthritis similar to human. Treatment with Duhuojisheng Decoction significantly down-regulated the expression level of tumor necrosis factor-α and significantly reduced the levels of whole blood leukocytes, and C-reactive protein. Our results suggest that the aged macaca fascicularis model of primary knee osteoarthritis is an effective animal model to simulate the occurrence and development of human primary knee osteoarthritis.Duhuojisheng decoction alleviates the progression of primary knee osteoarthritis probably by inhibiting the expression level of tumor necrosis factor-α and decreasing the levels of inflammatory factors in the articular cartilage. Additionally, the levels of whole blood leukocytes and C-reactive protein are important parameters for the prediction of curative effects.关键词
独活寄生汤/食蟹猴/自发性膝骨关节炎模型/肿瘤坏死因子α/C-反应蛋白/组织构建分类
医药卫生引用本文复制引用
陈巧玉,周鑫,扶世杰..独活寄生汤干预食蟹猴自发性膝骨关节炎模型炎症的作用[J].中国组织工程研究,2018,22(12):1866-1871,6.基金项目
四川省科技支撑计划项目(2014SZ0185) （2014SZ0185）
泸州市科技计划项目课题(2013LZLY-K59) （2013LZLY-K59）
泸州医学院-泸州医学院附属中医院联合专项项目(LYYK201312-10)the Science and Technology Support Program Project of Sichuan Province,No.2014SZ0185 （LYYK201312-10）
the Science and Technology Program of Luzhou,No.2013LZLY-K59 （）
the Joint Project of Luzhou Medical University&Affiliated Traditional Chinese Medicine Hospital of Luzhou Medical University,No.LYYK201312-10 （）
