中国组织工程研究2018，Vol.22Issue(12)：1883-1888,6.DOI:10.3969/j.issn.2095-4344.0807
白细胞介素17A在视神经损伤模型小鼠神经再生中的作用
Interleukin-17A facilitates optic nerve regeneration in a mouse model of optic nerve crush
摘要
Abstract
BACKGROUND: Interleukin-17A has been shown to play an important role in nerve regeneration following corneal injury. OBJECTIVE: To investigate the effect of interleukin-17A on mouse nerve regeneration after optic nerve injury.METHODS: The interleukin-17A-overexpressed adenovirus vector (AV-IL-17A-GFP) was constructed and purified. Adenoviruses with green fluorescent protein (AV-GFP) served as control group. PC12 cell lines were transfected with adenoviruses and the expression efficiency of IL-17A were detected by ELISA. The adenovirus was intravitreally injected into the mouse vitreous body, and the expression level of IL-17A in the optic nerve was determined by immunofluorescence. Afterwards, a mouse model of optic nerve injury was established to observe the nerve regeneration. RESULTS AND CONCLUSION: ELISA results showed that IL-17A was highly expressed in the PC12 cell lines transfected with AV-IL-17A-GFP compared with the control group.Immunofluorescence results revealed that IL-17A was expressed in vivo successfully.There was a significant increase in the number of regenerated axons in the AV-IL-17A-GFP group compared with the control group (P < 0.05). To conclude,AV-IL-17A-GFP can be expressed in the mature neurons in vivo and promote the axon regeneration after optic nerve crush injury in mice.关键词
白细胞介素17A/组织构建/实验动物模型/小鼠视神经损伤模型/中枢神经系统/细胞因子/腺病毒载体/ELISA/免疫荧光/视神经损伤/神经再生分类
医药卫生引用本文复制引用
李艳芬,叶晓阳,陈晓帆,张伟..白细胞介素17A在视神经损伤模型小鼠神经再生中的作用[J].中国组织工程研究,2018,22(12):1883-1888,6.基金项目
国家自然科学基金青年科学基金项目(81402600) （81402600）
深圳市科技研发资金知识创新计划基础研究项目(JCYJ20150403110829622)the National Natural Science Foundation of China for the Youth,No.81402600 （JCYJ20150403110829622）
the Knowledge Innovation and Basic Research Program of Shenzhen Science and Technology Research&Development Foundation,No.JCYJ20150403110829622 （）
