中国组织工程研究2018，Vol.22Issue(13)：2033-2038,6.DOI:10.3969/j.issn.2095-4344.0494
人脂肪干细胞及其外泌体的分离与鉴定
Isolation and identification of human adipose-derived stem cells and its exosomes
摘要
Abstract
BACKGROUND: Currently, mesenchymal stem cells have been widely explored and applied in scientific research field, and many studies suggest that the underlying mechanism of mesenchymal stem cells mainly relies on its exosomes. OBJECTIVE: To isolate and identify human adipose-derived stem cells and its exosomes, and to identify their biological characteristics. METHODS: Human adipose tissue was digested with collagenase l, and adipose-derived stem cells were isolated and purified. Immunophenotype, osteogenic and adipogenic abilities of adipose-derived stem cells were identified. Exosomes were isolated by using ultrafiltration method. Morphology of exosomes was observed by Nanosight and electron microscope. Expression of proteins in exosomes was detected by antibody array method. RESULTS AND CONCLUSION: Adipose-derived stem cells exhibited long spindle-like or fibroblast-like appearance, expressed CD73, CD44, CD90, CD105 and had the potential to differentiate into many tissues, including bone and adipose tissues. The exosomes had the similar size, with the diameter of 30-150 nm. They possessed many proteins including FLOT1, ICAM, ALIX, CD81, CD63, ANXA5, TSG101, and so on. Findings from the present study indicate the successful isolation of exosomes from human adipose-derived stem cells.关键词
外泌体/干细胞/间充质干细胞/脂肪干细胞分类
医药卫生引用本文复制引用
李洪超,金银鹏,王皙,李莉,王晓今,周荣,陈成伟,傅青春,程明亮..人脂肪干细胞及其外泌体的分离与鉴定[J].中国组织工程研究,2018,22(13):2033-2038,6.基金项目
南京军区医学创新重大课题(14ZX01) （14ZX01）
中国肝炎防治基金会-天晴肝病研究基金资助课题(TQGB20150104)the Medical Innovation Project of the Nanjing Military Region,No.14ZX01 （TQGB20150104）
the Chinese Foundation for Hepatitis Prevention and Control-Sunshine Foundation for Hepatitis,No.TQGB20150104 （）
