中国组织工程研究2018，Vol.22Issue(13)：2039-2044,6.DOI:10.3969/j.issn.2095-4344.0490
单唾液酸四己糖神经节苷脂最佳质量浓度诱导人脐带间充质干细胞向神经元样细胞的分化
Monosialotetrahexosyl ganglioside at an optimal concentration: inducing neuron-like differentiation of human umbilical cord mesenchymal stem cells
张鹏 1赵宗茂 2李建华 1刘辉 1刘永军 1李敏捷 1陈明伟 1申仑 1何磊1
作者信息
- 1. 邯郸市第一医院,河北省邯郸市 056000
- 2. 河北医科大学附属第二医院,河北省石家庄市 050000
- 折叠
摘要
Abstract
BACKGROUND: Studies have confirmed that monosialotetrahexosyl ganglioside can induce human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells, but little is reported on its optimal concentration. OBJECTIVE: To explore the optimal concentration of monosialotetrahexosyl ganglioside that induces human umbilical cord mesenchymal stem cells to differentiate into neuron-like cells in vitro. METHODS: Human umbilical cord mesenchymal stem cells were isolated by using collagenase digestion method, and after expansion, passage 3 cells were randomly allocated into five groups. When 70%-80% of cells were confluent, 50, 100, 150 and 200 mg/L monosialotetrahexosyl ganglioside induction solutions were added in corresponding experimental groups, while cells in the blank control group were cultured in the same volume of L-DMEM medium. Cell morphology was observed under inverted phase contrast microscope. Expression levels of microtubule-associated protein 2, neurofilament protein and glial fibrillary acidic protein were measured by using immunohistochemistry at 6 hours after induction. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells were isolated successfully and sub-cultured stably. These cells could express surface markers of mesenchymal stem cells. Monosialotetrahexosyl ganglioside at the optimal concentration of 150 mg/L was confirmed to induce the neuron-like differentiation of human umbilical cord mesenchymal stem cells, and differentiated cells could express microtubule-associated protein 2 and neurofilament protein as neuron-specific markers.关键词
干细胞/单唾液酸四己糖神经节苷脂/人脐带间充质干细胞/诱导分化/神经元样细胞分类
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张鹏,赵宗茂,李建华,刘辉,刘永军,李敏捷,陈明伟,申仑,何磊..单唾液酸四己糖神经节苷脂最佳质量浓度诱导人脐带间充质干细胞向神经元样细胞的分化[J].中国组织工程研究,2018,22(13):2039-2044,6.
