烟草科技2018，Vol.51Issue(4)：1-11,11.DOI:10.16135/j.issn1002-0861.2017.0361
烟草HD-ZIPⅣ转录因子NtPDF2基因的克隆及表达分析
Molecular cloning and expression analysis of HD-ZIP Ⅳ transcription factor gene NtPDF2 from Nicotiana tabacum
摘要
Abstract
To identify the biological functions of NtPDF2 and its mechanism in regulating trichome development in tobacco, NtPDF2 gene was cloned by homologous cloning strategy. The gene sequence, exon/intron structure, phylogenetic relationship and expression pattern of NtPDF2 were analyzed. The results showed that the full-length CDS sequence of NtPDF2 was 2 199 bp, which encoded 732 amino acids. The putative protein sequences of tobacco PDF2 were highly conserved, especially C-terminal which included three conserved domains (Homeobox domain, START domain and SAD domain) and one conserved LZ(Leucine zipper)motif. The gene structure of NtPDF2 was highly conserved, including 10 exons and 9 introns. Phylogenetic analysis revealed that NtPDF2 was evolved from NsylML1 gene. The expression pattern of NtPDF2 had an obvious spatio-temporal specificity. The transcription level of NtPDF2 significantly decreased under drought, darkness and phosphate starvation, while it did not change obviously under salt stress. Furthermore,GA, ABA, MeJA and topping treatments significantly induced the expression of NtPDF2, which suggested that NtPDF2 might participate in plant responses to various abiotic stresses and phytohormones.关键词
烟草/HD-ZIP Ⅳ转录因子/NtPDF2/非生物胁迫/激素应答Key words
Tobacco/HD-ZIP Ⅳ transcription factor/NtPDF2/Abiotic stress/Hormone response分类
农业科技引用本文复制引用
王姗姗,杨军,林福呈,孟祥宇,余涵,张剑锋,王中..烟草HD-ZIPⅣ转录因子NtPDF2基因的克隆及表达分析[J].烟草科技,2018,51(4):1-11,11.基金项目
中国烟草总公司烟草基因组计划重大专项项目"烟草转录因子基因芯片研制及重要转录因子表达调控研究"[110201401011(JY-11)]. （JY-11）
