烟草科技2018，Vol.51Issue(4)：12-19,8.DOI:10.16135/j.issn1002-0861.2017.0270
基于高通量测序的不同产地和部位自然醇化片烟真菌群落结构分析
High-throughput sequencing fungal community structures in aging tobacco strips from different growing areas and stalk positions
摘要
Abstract
To investigate the diversity of fungal community in tobacco strips during aging, the fungal ITS DNA in 12 samples of upper, middle and lower stalk leaves from four growing areas (Zhanyi in Yunnan, Changshun in Guizhou, Youxi in Fujian and Queshan in Henan) were sequenced by MiSeq. The results showed that: 1) A total of 1 155 operational taxonomic units (OTUs) were obtained from the 12 samples. Samples B2F and C3F from Youxi had the highest fungal community richness and the most abundant biodiversity, respectively. 2) Ascomycota was the dominant phyla in the 12 samples. Phoma, Alternaria and Monographella were the dominant genera in samples C3F and X2F from Zhanyi, samples B2F, C23F and X2F from Queshan and sample X23F from Changshun. Aspergillus was the dominant genera in the other 6 samples. 3) 121, 79 and 168 of OTUs were common to the samples of upper, middle and lower stalk leaves from the 4 areas. The number of reads from the common OTUs accounted for more than 70% of the total number of reads in each sample, except for the sample C3F from Youxi. The differences in fungal community structure from the same stalk position of different areas mainly reflected in the proportion of dominant genera.关键词
片烟/自然醇化/真菌/MiSeq/ITS/群落结构Key words
Tobacco strip/Aging/Fungus/MiSeq/ITS/Community structure分类
轻工纺织引用本文复制引用
陈善义,李菁菁,林俭,包可翔,范坚强,张瑞强,陈义强,陈少滨,何伟..基于高通量测序的不同产地和部位自然醇化片烟真菌群落结构分析[J].烟草科技,2018,51(4):12-19,8.基金项目
福建中烟工业有限责任公司科技项目"四段式气调醇化过程中各个节点烤烟叶面微生物的分离鉴定"(JSZX2014020) （JSZX2014020）
中国烟草总公司面上项目"四段式烟叶醇化及综合养护技术研究"(FJZYJH2012011). （FJZYJH2012011）
