烟草科技2018，Vol.51Issue(4)：20-27,8.DOI:10.16135/j.issn1002-0861.2017.0407
烟草黑胫病拮抗菌XF10的筛选与鉴定
Screening and identification of an antagonistic bacterium XF10 against tobacco black shank
摘要
Abstract
In order to quickly and effectively screen out antibacterial strains that have obvious antagonism against tobacco black shank, 300 soil samples were collected from the healthy plants'rhizosphere in serious tobacco black shank incidence areas. A strain, XF10, was obtained by rapid initial screening of pathogenic bacteria and confrontation culture. The appearance of XF10 colony was round with a smooth surface and neat edges. XF10 could produce orange pigment and was identified by morphological characteristics, biological phenotype identification, BIOLOG automatic microbiological identification system and 16S rDNA sequence analysis. The results showed that the homology of sequence between XF10 and several Pseudomonas chlororaphis strains in Pseudomonas reached 99%, therefore XF10 was identified as Pseudomonas chlororaphis. Both bacterial fluid and extracellular metabolite crude extract of XF10 possessed more than 80% inhibitory rate on Phytophthora parasitica var. nicotianae (Breda de Haan) Tucker. Moreover, the volatile metabolites of XF10 could inhibit the growth of pathogenic hyphae with an inhibition rate of 86%.关键词
烟草黑胫病菌/生防菌/拮抗作用Key words
Tobacco black shank/Antagonistic bacterium/Antagonism分类
农业科技引用本文复制引用
杨艺炜,黎妍妍,张安盛,周建云,李斌,王静,陈德鑫..烟草黑胫病拮抗菌XF10的筛选与鉴定[J].烟草科技,2018,51(4):20-27,8.基金项目
国家烟草专卖局(公司)重点科技项目"烟草疫霉与寄主识别过程与机制"(110201503008) （公司）
山东省现代农业产业技术体系烟草产业创新团队(SDAIT-25-03) （SDAIT-25-03）
四川省烟草专卖局(公司)重点科技项目"四川烟草主要病害绿色防控技术集成示范与推广"(SCYC201604)、"四川烟草叶部斑点病害绿色防控技术研究与示范应用"(SCYC201703) （公司）
贵州省烟草专卖局(公司)重点科技项目"贵阳烟草主要病虫草绿色防控技术集成示范与推广"(201606). （公司）
