烟草科技2018，Vol.51Issue(4)：41-45,5.DOI:10.16135/j.issn1002-0861.2017.0303
连续流动法测定烟草中还原糖含量的方法优化
Optimization of a continuous flow method to determine reducing sugar content in tobacco
摘要
Abstract
In order to improve the accuracy to determine reducing sugar contents in tobacco by continuous flow method, the test parameters described in the domestic tobacco industrial standard YC/T 159—2002 were optimized via an AA3 continuous flow analyzer. The optimized conditions were shown as follows: 0.20 mol/L hydrochloric acid solution was used as the activated water in red/red pipeline, the concentration of sodium hydroxide solution was reduced to 0.35 mol/L, and the temperature of the heating tank was lowered to 79 ℃. The results showed that: 1) Adopting the optimized method, the correlation coefficient of the standard curve was 0.999 9, the limit of detection and the limit of quantitation were 0.006 3% and 0.021 0%, respectively. The relative standard deviations (RSDs) of intra- and inter-day were less than 5% and the spiked recoveries ranged from 96.88% to 102.86%. 2) F test indicated that there was no significant difference between the results determined by the optimized method and the standard method. The optimized method could continuously and accurately determine the content of reducing sugar in tobacco by 60 cups(40 cups more than the standard method).关键词
连续流动法/烟草/还原糖/方法优化Key words
Continuous flow method/Tobacco/Reducing sugar/Method optimization分类
轻工纺织引用本文复制引用
何声宝,罗安娜,王英元,冯晓民,张威,范月月,刘楠..连续流动法测定烟草中还原糖含量的方法优化[J].烟草科技,2018,51(4):41-45,5.基金项目
中国烟草总公司科技重大专项项目"提高细支卷烟质量稳定性的关键工艺技术研究"(522016AA0190.2). （522016AA0190.2）
