烟草科技2018，Vol.51Issue(4)：53-59,80,8.DOI:10.16135/j.issn1002-0861.2017.0333
GC-MS/MS法分析常用食品和卷烟烟气中的氨基甲酸乙酯
Determination of ethyl carbamate in daily foods and cigarette smoke by gas chromatography coupled tandem mass spectrometry
摘要
Abstract
For quantifying trace levels of ethyl carbamate (EC) in daily foods and cigarette smoke, a reverse-phase solid phase extraction cleanup method coupled with solid phase reverse extraction and gas chromatography/tandem mass spectrometry was developed by adopting pulse splitless injection mode. After extracting EC from samples by using water as a solvent, the extract was cleaned up by C18reverse-phase solid phase extraction column, then was reversely extracted by diatomite solid phase extraction column with dichloromethane. The eluent was detected with gas chromatography-tandem mass spectrometry after concentration with deuterium-labeled EC as internal standard for quantitation. The results showed that: 1) Under optimal condition, the calibration curve was linear (R2=0.999 9) when the concentration of EC was in the range of 0.75-48.00 μg/L, the limits of detection and quantitation were 0.025 9 and 0.086 3 μg/kg, respectively. The recoveries of different sample matrix ranged from 91.56% to 112.22% with relative standard deviations of 4.24%-7.97%. 2) The results from surveyed samples indicated that the contents of EC in several daily foods,including alcoholic beverage (yellow rice wine, white spirit, beer and red wine), cooking wine, soy sauce, yoghourt and fermented bean curd ranged from 5.17-523.66 μg/kg, and the release of EC in cigarette smoke ranged from 0.33 to 1.20 ng/cig. With higher sensitivity and better applicability, this method could be used to detect trace EC in other daily foods and cigarette smoke.关键词
反相固相萃取净化/固相反萃取/气相色谱/串联质谱(GC-MS/MS)/日常食品/卷烟/烟气/氨基甲酸乙酯Key words
Reverse solid phase extraction cleanup/Solid phase reverse extraction/GC-MS/MS/Daily food/Cigarette/Smoke/Ethyl carbamate分类
轻工纺织引用本文复制引用
廖惠云,张华,秦艳华,庄亚东,熊晓敏,尤晓娟,朱莹,张媛,王晨辉..GC-MS/MS法分析常用食品和卷烟烟气中的氨基甲酸乙酯[J].烟草科技,2018,51(4):53-59,80,8.基金项目
国家烟草专卖局资助项目"卷烟主流烟气中氨基甲酸乙酯的测定"(2013QB001). （2013QB001）
