眼科新进展2018，Vol.38Issue(5)：407-411,5.DOI:10.13389/j.cnki.rao.2018.0095
高糖抑制角膜缘干细胞间质性及其迁移能力的研究
High glucose suppresses the corneal limbal stem cell migration and changes the cell phenotype
摘要
Abstract
Objeetive To explore the influence of extracellular high glucose on the proliferation,migration and biomarkers of corneal limbal stem cells.Methods Establishment of a model of high glucose in cultured human limbal stem cells to observe and investigate the effects of extracellular high glucose on the proliferation and migration of corneal limbal stem cells by immunoflurescence,CCK-8 and Transwell assay,respectively.Totally 16 SPF rats were collected and induced diabetic model by streptozotocin as the high-glucose group,and the normal rats of the same age served as the control group.Corneal epidermises of rats in both groups were scraped to observe the repair of corneal epithelium.And the corneas were treated with HE staining and immunohistochemical staining to detect the expression of biomarkers of corneal limbal stem cells and the modality changes of cells.Results The proliferation rate of human limbal epithelial cells was significantly decreased when exposed to high glucose,and the rate at 24 h,48 h and 72 h was 0.728,0.345 and 0.395,respectively,which was markedly lower than that in the control group,with a significant difference (P ＜ 0.05);meanwhile the cell migration rate of the high-glucose group was 17.6％ at 48 h,which was significantly slower than that of the control group (100％).And the inhibition was accompanied by the decreased expression of β-catenin and vimentin.Furthermore,the expression levels of β-catenin and vimentin mRNA and protein were down-regulated,with abnormal location,in the high-glucose group.And diabetic rats had poor corneal epithelial healing.The epithelial layer became thinner and the structures were disorganized in diabetic rats through HE staining.The immunohistochemical assay revealed the expression of β-catenin and vimentin of cornea limbal stem cells was down-regulated in high-glocose group when compared with the control group.Conclusion High glucose can significantly inhibit the proliferation and migration of cornea limbal stem cells,and its main damage mechanism is correlated with the abnormalities of β-catenin and vimentin.关键词
角膜缘干细胞/β-catenin/vimentin/糖尿病角膜病变Key words
corneal limbal stem cells/β-catenin/vimentin/diabetic keratopathy分类
医药卫生引用本文复制引用
马文贝,冯松福,韦诗羽,陈慧,梁丽芳,钟彦彦,陆晓和..高糖抑制角膜缘干细胞间质性及其迁移能力的研究[J].眼科新进展,2018,38(5):407-411,5.基金项目
国家自然科学基金面上项目(编号:81371067) （编号:81371067）
广东省自然科学基金项目(编号:S2013040013713) National Natural Science Foundation of China (No:81371067) （编号:S2013040013713）
Natural Science Foundation of Guangdong Province(No:S2013040013713) （No:S2013040013713）
