眼科新进展2018，Vol.38Issue(5)：412-415,420,5.DOI:10.13389/j.cnki.rao.2018.0096
光预适应对小鼠视网膜光感受器细胞光损伤的保护作用
Protective effects of light preconditioning on retinal photoreceptor cells aganist light damage
摘要
Abstract
Objective To investigate the protective role of light preconditioning in retinal photoreceptor cells and the underlying mechanisms after light damage.Methods Together 42 BALB/c mice were randomly divided into normal control group,light damage group,light preconditioning + light damage group and light preconditioning group.Mice in the light damage group were exposed to 4000 Lux intensity of cool white fluorescent light for 4 h continually;mice in the light reconditioning + light damage group were maintained in 600 Lux cyclic bright light for preconditioning (12 h ON and 12 h OFF) for 6 days,and then exposed to 4000 Lux bright-light for the induction of the damage;mice in the light reconditioning group were maintained in 600 Lux cyclic bright light for preconditioning (12 h ON and 12 h OFF) for 2 days,4 days,6 days.Then the function and morphology of retinal photoreceptor cells were detected by flash electroretinogram (FERG) and histopathological examination in the normal control group,light damage group and light preconditioning + light damage group,while real-time polymerase chain reaction (PCR) was used to detect the mRNA relative expression of leukemia inhibitory factor (LIF) and Western blot analysis was used to detect the phosphoprotein relative expression of signal transducer and activator of transcription 3(STAT3) in the light preconditioning group.Results FERG results showed the amplitudes of scotopic ERG a wave of the light damage group were decreased when compared with the normal control group,with significant difference (P =0.000).Compared with the light damage group,the amplitudes of scotopic ERG a wave of the light preconditioning + light damage group were increased significantly (P =0.000).Histopathological examination results showed that the number of photoreceptor nuclei in the light damage group was decreased compared with the normal control group,and the difference was statistically significant (P =0.000).Compared with light damage group,the number of photoreceptor nuclei in light preconditioning + light damage group was increased significantly (P =0.000).Real-time PCR results showed light preconditioning enhanced the LIF mRNA relative expression in a time-dependent manner (F=128.776,P =0.000).Western blot results showed light preconditioning up-regulated the phosphoprotein relative expression of STAT3 protein in a time-dependent manner (F =73.493,P =0.000).Conelusion Light preconditioning can protect retinal photoreceptor cells against light damage which may results from the activation of LIF/STAT3 signaling pathway.关键词
光预适应/光感受器/白血病抑制因子/光损伤Key words
light preconditioning/photoreceptor/leukemia inhibitory factor/light damage分类
医药卫生引用本文复制引用
董淑倩,刘双珍,李秋明..光预适应对小鼠视网膜光感受器细胞光损伤的保护作用[J].眼科新进展,2018,38(5):412-415,420,5.基金项目
国家自然科学基金联合基金项目(编号:U1304812) （编号:U1304812）
河南省医学科技攻关计划普通项目(编号:201602080)Joint Project of National Natural Science Foundation of China(No:U1304812) （编号:201602080）
General Project of Medical Science and Technology of Henan Province(No:201602080) （No:201602080）
