眼科新进展2018，Vol.38Issue(5)：430-433,4.DOI:10.13389/j.cnki.rao.2018.0100
骨膜蛋白在眼睑基底细胞癌相关成纤维细胞与眼睑正常成纤维细胞中的表达差异
Expression differences and significance of periostin in eyelid basal cell carcinoma associated fibroblasts and-normal fibroblasts
摘要
Abstract
Objective To investigate the expression differences and significance of periostin (PN) in eyelid basal cell carcinoma associated fibroblasts (BCAFs) andnormal fibroblasts (NFs) after separation,culture,purification and identification.Methods The third generation of purified BCAFs and NFs was selected,and the concentrations of cell suspensions were modulated to 20 × 106 L-1 by trypsin,and then the cell suspension were seeded and cultured in 6-well plate by 2 mL per well.The cell culture supernatants were collected when BCAFs and NFs were cultured by serum-free medium for 48 h,then the content of PN in cell culture supernatants from BCAFs and NFs was detected by enzyme-linked immunosorbent assay (ELISA).The glass coverslips were placed at the bottom of the 6-well plate to make cell slides,and then the expression of PN in BCAFs and NFs cells were tested by immunofluorescence staining.Results ELISA showed that the content of PN in cell culture supernatants from BCAFs and NFs was (9.26 ± 2.35) μg · L-1 and (2.57 ± 0.41) μg · L-1.And the expression level of PN in BCAFs tested by immunofluorescence staining technology was higher than that in NFs cells,and the differences were statistically significant (all P ＜ 0.05).Conclusion The expression and secretion of PN in the eyelid BCAFs were highly enhanced when compared with NFs,suggesting that periostein may promote or inhibit the occurrence and development of the eyelid basal cell carcinoma in the microenvironment of the eyelid basal cell carcinoma.关键词
骨膜蛋白/眼睑基底细胞癌/癌相关成纤维细胞/正常成纤维细胞Key words
periostin/eyelid basal cell carcinoma/carcinoma associated fibroblasts/normal fibroblasts分类
医药卫生引用本文复制引用
赵佐芳,马善春,田翠玲,连刚,涂静,王定松,李维,王大庆,王宁,雷小平,朱大勇,张艳红,税民鸿,贾前伟,程阳..骨膜蛋白在眼睑基底细胞癌相关成纤维细胞与眼睑正常成纤维细胞中的表达差异[J].眼科新进展,2018,38(5):430-433,4.基金项目
四川省卫生和计划生育委员会科研课题(编号:17PJ549)Health Development Science Foundation of Sichuan Province(No:17PJ549) （编号:17PJ549）
