中国肺癌杂志2018，Vol.21Issue(5)：351-357,7.DOI:10.3779/j.issn.1009-3419.2018.05.01
肿瘤微环境中脂肪细胞对肺腺癌A549细胞生物学行为影响的初步研究
Preliminary Study on the Effect of Adipocytes on the Biological Behaviors of Lung Adenocarcinoma A549 Cells in Tumor Microenvironment
摘要
Abstract
Background and objective Adipocytes in the tumor microenvironment may provide the metabolic fuel or signal transduction through media and other means to promote a variety of malignant proliferation and invasion, of tumor cells, but their role in lung cancer progression is still unclear. The purpose of this study was to investigate the effect of adipo-cytes on lung cancer cell biology. Methods 3T3-L1 pre-adipocytes were induced into mature adipocytes. The cell morphol-ogy was observed by microscopy and Oil Red O staining. MTT assay, colony formation assay, wound-healing and Transwell methods were used to detect lung cancer cell proliferation, migration and invasion ability. The content of triglyceride in cells was determined by colorimetry. Results The morphology of lung adenocarcinoma A549 cells became more slender after co-culture with mature adipocytes, and the proliferation and cloning ability were signiffcantly enhanced (P<0.05). In addition, mature adipocytes can also promote the migration ability (P<0.05), invasion ability (P<0.01) and accumulation of intracellular lipid (P<0.05) of A549 cells. Conclusion These findings suggested that adipocytes in tumor microenvironment can promote the proliferation, migration and invasion of lung adenocarcinoma A549 cells, which may be related to lipid metabolism.关键词
肺肿瘤/脂肪细胞/细胞增殖/迁移/侵袭Key words
Lung neoplasms/Adipocyte/Cell proliferation/Migration/Invasion引用本文复制引用
张航,李晶晶,曹亚男,董翔,高聪,李烦繁..肿瘤微环境中脂肪细胞对肺腺癌A549细胞生物学行为影响的初步研究[J].中国肺癌杂志,2018,21(5):351-357,7.基金项目
本研究受国家自然科学基金(No.81402427)、安徽省自然科学基金(No.1408085QH165)和教育部高等学校博士点专项科研基金(No.20133420120001)资助This study was supported by the grants from National Natural Science Foundation of China(No.81402427),Provin-cial Natural Science Foundation of Anhui(No.1408085QH165)and the Ministry of Education Fund to Specialized Research for the Doctoral Program of Higher Education(all to Fanfan LI)(No.20133420120001). （No.81402427）
