中国临床药理学杂志2018，Vol.34Issue(6)：663-666,4.DOI:10.13699/j.cnki.1001-6821.2018.06.016
微小RNA在中国汉族人血浆中的分布研究
Study on plasma distribution of microRNA in Chinese Han population
摘要
Abstract
Objective To establish a method for extracting and detecting circulating microRNAs(miRNAs) in plasma and to detect the distribution of miR-192,miR-206 and miR-613 in Chinese Han people.Methods The total RNA was extracted from 116 healthy subjects plasma by Trizol LS and then tested by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method.The expression level of circulating miR-192,miR-206 and miR-613 was standardized by eel-39.Results The △Ct values of circulating miR-192,miR-206 and miR-613 in 116 healthy subjects plasma were 14.01 ± 4.19,17.63 ±4.42 and 19.77 ±4.68,respectively.The expression of plasma circulating miR-192 was significantly higher than miR-206 and miR-613 (P ＜0.05).After grouped by quarrles,the differences of circulating miR-192,miR-206 and miR-613 among four groups were statistically significant (P ＜ 0.05).Conclusion The expression of circulating miR-192,miR-206 and miR-613 in Chinese Han people was distributed normally and showed significant differences.关键词
微小RNA-192/微小RNA-206/微小RNA-613/分布Key words
miRNA-192/miRNA-206/miRNA-613/distribution分类
医药卫生引用本文复制引用
靳高凤,李西,朱丹丹,丁薇,张红,夏春华,李新华,熊玉卿..微小RNA在中国汉族人血浆中的分布研究[J].中国临床药理学杂志,2018,34(6):663-666,4.基金项目
国家自然科学基金资助项目(81673506) （81673506）
江西省研究生创新专项基金资助项目(YC2015-B005) （YC2015-B005）
