摘要
Abstract
Objective To investigate the protective effects of panaxadiol saponins combined with edaravone on cardiopulmonary injury rats with endotoxic shock.Methods Forty Wistar rats were randomly divided into sham operation group,model group,positive control group and test group,each group 10 rats.The model group,positive control group and test group were injected with Escherichia coli lipopolysaccharide (LPS)to establish the endotoxic shock rat model,sham operation group was injected with normal saline 1 mL.After modeling,sham operation group and model group were injected with normal saline 0.5 mL through femoral vein,positive control group was injected with dexamethasone 3 mg · kg-1 through femoral vein,test group was injected with panaxadiol saponins 45 mg · kg-1 + edaravone 3 mg · kg-1 through femoral vein.The levels of serum enzyme and oxidative stress in lung tissues were measured 4 h after drug intervention,the expression of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in lung tissue of each group were detected by immunohistochemistry,hematoxylin-eosin (HE) staining was used to observe the morphological changes of lung tissue in rats.Results After 4 h intervention,the levels of serum creatine kinase (CK),aspartate aminotransferase (AST),lactate dehydrogenase (LDH) in model group were (1025.12±368.45),(526.48±201.52),(634.58 ± 267.41) U · L-1,and were (562.34 ± 211.26),(286.45±232.15),(359.48±105.36)U · L-1 in positive group,which were (389.45 ± 268.47),(252.36 ± 124.51),(298.46 ± 169.74)U · L-1 in test group.The levels of above index in positive group and test group had significant difference with those in model group(P ＜0.01).The levels of MMP-2 and TIMP-2 in model group were 0.24 ±0.06 and 0.15 ±0.04 in lung tissue,and were 0.09 ±0.02 and 0.14 ±0.02 in sham operation group and 0.16 ±0.04 and 0.15±0.04 in positive control group,and were 0.18 ±0.05,0.16 ±0.03 in experimental group.The difference between model group and sham operation group was statistically significant (all P ＜ 0.01).The difference between experimental group and positive control group and model group was statistically significant (all P ＜0.01).Conclusion Panaxadiol saponins combined with edaravone can reduce the oxidative stress and inflammatory degree of lung injury in endotoxin shock rats,down-regulate the expression of MMP-2 in lung tissue,and also protect the myocardium.关键词
人参二醇皂苷/依拉达奉/内毒素休克大鼠/心肺保护Key words
panaxadiol saponins/edaravone/endotoxin shock rats/cardiopulmonary protection分类
医药卫生
