中国临床药理学杂志2018，Vol.34Issue(6)：695-697,3.DOI:10.13699/j.cnki.1001-6821.2018.06.025
四逆散干预创伤后应激障碍及睡眠障碍大鼠海马的超微结构研究
Study on intervene function of Sinisan with the ultra structure of hippo-campus in rats with sleep disorder induced by post traumatic stress disorder
摘要
Abstract
Objective To study on intervene function of Sinisan with the ultra structure of hippo-campus in rats with sleep disorder induced by post traumatic stress disorder.Methods Fifty SD rats were divided equally into five groups:blank control group,model group,negative control group,positive control group and experimental group.The control blank group did not copy the model,do not receive drugs,the normal feeding.Model group,repetitive post-traumatic stress disorder model was induced by current stimulation,but not receive drugs.Negative control group,equal volume of 0.9％ NaCl.Positive control group (paroxetine hydrochloride 0.42 mg ·mL-1) and experimental group (Sinisan,containing crude drug 0.24 g · mL-1).It given to drugs 1 h before the model establishment.The rats were administered with 10 mL · kg-1,once a day,for a total of 7 d.The changes of ultra structure of CA1 and CA3 area in hippo-campus with rats were observed by transmission electronmicroscope.Results In the blank control group,the hippocampal neurons were clearly defined,and the synaptic space was obvious in the CA1 and CA3 area.Compared with the blank control group,in the model group,the synaptic structure of hippocampal neurons was significantly changed,the synaptic vesicles were reduced and the synaptic structure was not clear in the CA1 and CA3 area.Compared with the model group,the negative control group was same too,which indicated that there was no significant stress effect on the rats after intragastric administration of saline.Compared with the negative control group,positive control group and experimental group hippocampal neurons synapse structure was restored,and the two groups were similar in the CA1 and CA3 area.Conclusion Transmission electronmicroscope was used to study the ultra structure of CA1 and CA3 in rats hippo-campus with characteristic difference.关键词
创伤后应激障碍/四逆散/透射电镜/海马组织Key words
post traumatic stress disorder/Sinisan/transmission electronmicroscope/hippocampus japonicus分类
医药卫生引用本文复制引用
李越峰,边甜甜,司昕蕾,牛江涛,曹瑞,严兴科..四逆散干预创伤后应激障碍及睡眠障碍大鼠海马的超微结构研究[J].中国临床药理学杂志,2018,34(6):695-697,3.基金项目
国家自然科学基金资助项目(81460611) （81460611）
国家教育部科学技术研究重点基金资助项目(212186) （212186）
甘肃省自然科学基金资助项目(2010:1010RJZA212 （）
2014:145RJZA076) （）
甘肃省财政厅高校基本科研业务费专项基金资金项目(2013-2) （2013-2）
兰州市科技计划基金资金项目(2014-1-188) （2014-1-188）
甘肃省中医药管理局科研课题基金资金项目(GZK-2015-57) （GZK-2015-57）
